Effects of Banxia Xiexin Decoction () on Cisplatin-Induced Apoptosis of Human A549 Lung Cancer Cells.

Ha-rim Kim; Guem-san Lee; Mi-seong Kim; Do-gon Ryu; Hong-seob SO; Hyoung-chul Moon; Young-rae Lee; Sei-hoon Yang; Kang-beom Kwon

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Effects of Banxia Xiexin Decoction () on Cisplatin-Induced Apoptosis of Human A549 Lung Cancer Cells.

Author: Ha-Rim KIM ¹ ; Guem-San LEE ² ; Mi-Seong KIM ¹ ; Do-Gon RYU ³ ; Hong-Seob SO ¹ ; Hyoung-Chul MOON ⁴ ; Young-Rae LEE ¹ ; Sei-Hoon YANG ⁵ ; Kang-Beom KWON ⁶
Author Information

1. Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan, Jeonbuk, 54538, South Korea.
2. Department of Herbology, Wonkwang University School of Korean Medicine, Iksan, Jeonbuk, 54538, South Korea.
3. Department of Korean Physiology, Wonkwang University School of Korean Medicine, Iksan Jeonbuk, 54538, South Korea.
4. Institute of Customized Physical Therapy, Gwanju, 62279, South Korea.
5. Department of Internal Medicine, Wonkwang University School of Medicine, Iksan, Jeonbuk, 54538, South Korea.
6. Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan, Jeonbuk, 54538, South Korea. desson@wku.ac.kr.
Publication Type:Journal Article
Keywords: A549 cells; Banxia Xiexin Decoction; Chinese medicine; anticancer; apoptosis; cisplatin; synergism
MeSH: A549 Cells; Apoptosis; drug effects; Apoptosis Regulatory Proteins; metabolism; Caspase Inhibitors; pharmacology; Cisplatin; pharmacology; DNA Fragmentation; drug effects; Humans; Plant Extracts; pharmacology
From: Chinese journal of integrative medicine 2018;24(6):436-441
CountryChina
Language:English
Abstract:
OBJECTIVETo examinie the synergistic effects of Banxia Xiexin Decoction (, Known as Banhasasim-tang in Korean) extract (BXDE) on cisplatin-induced cytotoxicity in the A549 human lung cancer cell lines.
METHODSA549 cells were treated with varying concentrations (50-200 μg/mL) of cisplatin and BXDE alone or in combination for 96 h. We used 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan assay and flow cytometry to analyze cell viability and apoptosis, respectively.
RESULTSThe exposure of cells to cisplatin and BXDE alone or in combination decreased cell viability dose- and time-dependently (P<0.05), which was found to be mediated by the apoptotic pathway as confirmed by the increase in the annexin V/propidium iodide- stained cell population and a ladder pattern of discontinuous DNA fragments. Furthermore, the apoptosis was inhibited by the pan-caspase inhibitor, benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (z-VAD-FMK).
CONCLUSIONSBXDE significantly potentiated apoptotic effects of cisplatin in A549 cells. Moreover, apoptosis induced by BXDE might be the pivotal mechanism mediating its chemopreventative action against cancer.