Long chain non-coding RNA MALAT-1 gene knockdown inhibits growth and migration and promotes apoptosis of human laryngeal squamous cell carcinoma Hep-2 cells .
- Author:
Yuefeng HAN
1
;
Deshang CHEN
1
;
Hui LI
1
;
Xiaomin WANG
1
;
Mingjie ZHANG
1
;
Yang YANG
1
Author Information
- Publication Type:Journal Article
- Keywords: MALAT1; apoptosis; laryngeal neoplasms; long chain non coded RNA; migration and invasion
- From: Journal of Southern Medical University 2018;38(8):923-930
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of knocking down long chain non-coding RNA MALAT-1 gene on the biologicalbehaviors of human laryngeal squamous cell carcinoma Hep-2 cells.
METHODSWith immortalized nasopharyngeal epithelial(NPE) cell line NP-69 as the reference, MALAT1 expression in FaDu, Hep-2 and nasopharyngeal carcinoma CNE-2Z cells weredetected using real-time PCR. Hep-2 cells were transfected with shmalat1 lentivirus and the expression of MALAT1 wasdetected. MTT assay, flow cytometry, Transwell assay and M Atrigel invasiveness test were used to evaluate the effect ofMALAT-1 knockdown on the proliferation, cell cycle, cell apoptosis, migration, and invasiveness of Hep-2 cells.
RESULTSCompared with NP-69 cells, Hep-2 cells, FaDu cells, and CNE-2Z cells all showed significantly increased MALAT-1expression. In Hep-2 cells, knockdown of MALAT-1 significantly inhibited the cell proliferation, increased the cell percentagein S phase ( < 0.01), decreased the cell percentage in G2/M phase ( < 0.01), and attenuated the migration and invasiveness of thecells.
CONCLUSIONSMALAT-1 is over-expressed in laryngeal squamous cell carcinoma, and knocking down MALAT-1 gene cansignificantly suppress the proliferation, invasion and migration and promotes apoptosis of the cancer cells.
