Application of atomic force microscopy-based single molecule force spectroscopy in G-quadruplex studies.
10.12122/j.issn.1673-4254.2018.09.14
- Author:
Wenjing LIU
1
;
Tong SUN
1
;
Ping ZHANG
1
;
Lin LI
2
;
Junhong LV
1
;
Bin LI
1
Author Information
1. Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
2. School of Science, Ningbo University, Ningbo 315211, China.
- Publication Type:Journal Article
- Keywords:
G-quadruplex;
atomic force microscopy;
single molecule force spectroscopy;
telomeres
- From:
Journal of Southern Medical University
2018;38(9):1107-1114
- CountryChina
- Language:Chinese
-
Abstract:
Telomere plays a crucial role in the physiological and pathological processes of cells. At the end of the telomere, the single-stranded DNA repeat sequence rich in guanine (G) folds in the presence of monovalent metal ions such as Na or K to form a G-quadruplex structure. This structure can not be extended by telomerase and inhibits the activity of telomerase, thus becoming a potential anticancer target. Stabilizing the formation of DNA G-quadruplex structures by small molecule ligands has become a new strategy for designing many anticancer drugs, and studying the interaction strength of these small molecule ligands with G-quadruplex is thus of particular importance for screening highly effective anticancer drugs. Single molecule force spectroscopy enables direct measurement of the interaction between small molecule ligands and G-quadruplexes. This review highlights the advances of single-molecule force spectroscopy based on atomic force microscopy in the study of the G quadruplex structure and its interaction with small molecule ligands, and summarizes the application and development trend of single molecule force spectrum technology in G quadruplex.
