Phytochemical characterization of polyphenolic compounds with HPLC-DAD-ESI-MS and evaluation of lipid-lowering capacity of aqueous extracts from Saharan plant Anabasis aretioides (Coss & Moq.) in normal and streptozotocin-induced diabetic rats.

Omar Farid; Farid Khallouki; Morad Akdad; Andrea Breuer; Robert Wyn Owen; Mohamed Eddouks

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Phytochemical characterization of polyphenolic compounds with HPLC-DAD-ESI-MS and evaluation of lipid-lowering capacity of aqueous extracts from Saharan plant Anabasis aretioides (Coss & Moq.) in normal and streptozotocin-induced diabetic rats.

Author: Omar FARID ¹ ; Farid KHALLOUKI ^{2
,

3} ; Morad AKDAD ¹ ; Andrea BREUER ⁴ ; Robert Wyn OWEN ⁴ ; Mohamed EDDOUKS ⁵
Author Information

1. Team of Physiology & Endocrine Pharmacology, Faculty of Sciences and Techniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, Errachidia 52000, Morocco.
2. Team of Physiology & Endocrine Pharmacology, Faculty of Sciences and Techniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, Errachidia 52000, Morocco
3. Division of Preventive Oncology, National Center for Tumor Diseases, Im Neuenheimer Feld 460, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 581, Heidelberg, Germany.
4. Division of Preventive Oncology, National Center for Tumor Diseases, Im Neuenheimer Feld 460, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 581, Heidelberg, Germany.
5. Team of Physiology & Endocrine Pharmacology, Faculty of Sciences and Techniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, Errachidia 52000, Morocco. Electronic address: mohamed.eddouks@laposte.net.
Publication Type:Journal Article
Keywords: 1,1-Diphenyl-2-picrylhydrazyl; Anabasis aretioides; Antioxidant activity; Lipid metabolism; Phytochemical analysis; Polyphenolic compounds
MeSH: Animals; Antioxidants; administration & dosage; Chenopodiaceae; chemistry; Cholesterol; blood; Chromatography, High Pressure Liquid; Diabetes Mellitus, Experimental; blood; drug therapy; Humans; Hypolipidemic Agents; administration & dosage; chemistry; Male; Phytochemicals; administration & dosage; chemistry; Plant Extracts; administration & dosage; chemistry; Polyphenols; administration & dosage; chemistry; Rats; Rats, Wistar; Streptozocin; Tandem Mass Spectrometry; Triglycerides; blood
From: Journal of Integrative Medicine 2018;16(3):185-191
CountryChina
Language:English
Abstract:
OBJECTIVEAnabasis aretioides (Coss & Moq.), a Saharan plant belonging to Chenopodiaceae family, is widely distributed in semi-desert areas from the Tafilalet region of Morocco. This plant is extensively used by local population against diabetes and cardiovascular disorders. The purpose of the study was to investigate the effect of the aqueous A. aretioides extract on lipid metabolism in normal and streptozotocin (STZ)-induced diabetic rats and to identify the polyphenolic compounds present. In addition, the in vitro antioxidant activity of the aqueous A. aretioides extract was also evaluated.
METHODSThe effect of an aerial part aqueous extract (APAE) of A. aretioides (5 mg/kg of lyophilized A. aretioides APAE) on plasma lipid profile was investigated in normal and STZ-induced diabetic rats (n = 6) after once daily oral administration for 15 days. The aqueous extract was tested for its 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity. Polyphenolic compounds in the extracts were definitively characterized by high-performance liquid chromatography-diode array detection-electrospray ionization-mass spectrometry.
RESULTSIn diabetic rats, oral administration of A. aretioides APAE provoked a significant decrease in both plasma cholesterol and triglyceride levels from the first to the second week (P < 0.01). A significant decrease on plasma triglyceride levels was also observed in normal rats (P < 0.01), where the reduction was 53%. In addition, the phytochemical analysis revealed the presence of 12 polyphenolic compounds. Moreover, according to the DPPH radical-scavenging activity, the aqueous extract showed an in vitro antioxidant activity.
CONCLUSIONAqueous A. aretioides APAE exhibits lipid-lowering and in vitro antioxidant activities. Many polyphenols were present in this extract and these phytoconstituents may be involved in the pharmacological activity of this plant.