Effect of Coagulation and Platelet Activation on Tumor-Associated Hypercoagulable State in Lymphoma-Bearing Mice.
- VernacularTitle:淋巴瘤荷瘤小鼠凝血状态与血小板活化对肿瘤高凝状态的影响
- Author:
Miao ZHU
1
;
Qing-Qing SHI
2
;
Xin SUN
2
;
Jun NI
2
;
Wei WU
2
;
Lian-Jun SHEN
2
;
Wei JI
2
;
Kai-Lin XU
1
;
Jian GU
3
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood Coagulation; Lymphoma; Mice; Mice, Inbred BALB C; P-Selectin; Platelet Activation; Thrombophilia
- From: Journal of Experimental Hematology 2018;26(2):427-431
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the coagulation abnormality and tumor-associated hypercoagulable state in lymphoma-bearing mice by measuring the changes in coagulation indices (D-D, vWF, TF) and platelet activation indices (P-selectin, GPIIbIIIa).
METHODSThe mouse model with lymphoma was established by the subcutaneous injection of 38B9 lymphoma cells into BALB/c mice, and the tumor formation was evaluated by using MRI and B ultrasonography. The D-D, vWF and TP levels of blood samples from inner canthal vein of tumor-bearing mice on 1 d, 14 d and 21 d were detected by using ELISA, the platelet activation indices (P-selectin, GPIIbIIIa) were detected by using flow cytometry.
RESULTSThe lymphoma-bearing mouse model was successfully established. The levels of D-D, vWF and TF as well as platelet activation indices P-selectin and GPIIbIIIa in the peripheraI blood were significantly higher than those of control group (P<0.05).
CONCLUSIONLymphoma-bearing mice showed abnormalities of coagulation and platelet activation, which relates with the tumor hypercoagulable state in lymphoma-bearing mice.
