Blocking Two Signals of Immuno-Activated T Cells from Antigen Presenting Cells Can Prevent Chronic GVHD of Murine.
- VernacularTitle:阻断CD4+T细胞活化的双信号可预防小鼠慢性GVHD
- Author:
Yuan-Bin WU
1
;
Kun-Yuan GUO
2
;
Guo-Zheng WANG
1
;
Xi-Ping DAI
1
Author Information
- Publication Type:Journal Article
- MeSH: Abatacept; Animals; Antigen-Presenting Cells; Antigens, CD; Antigens, Differentiation; CTLA-4 Antigen; Chronic Disease; Graft vs Host Disease; prevention & control; Immunoconjugates; Mice; Mice, Inbred C57BL; T-Lymphocytes
- From: Journal of Experimental Hematology 2018;26(2):557-562
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of blocking TCR-CD3 and B7-CD28 signals on immune function of mice with chronic GVHD by using TJU103 and CTLA4-Ig.
METHODSOn the basis of foregoing murine model of chronic GVHD, according to interference modes after infusion 6×10 spleen cells of donor mice, the recipients were divided into 5 groups: blank control, cGVHD, TJU103 interference, CTLA4-Ig interference and TJU103+CTLA4-Ig interference groups. The score of clinical manifestation and tissue histopathology were used to evaluate the effects of all the interferences on chronic GVHD.
RESULTSTJU103 and CTLA4-Ig could not influence the formation of the mouse chimera. The analysis of Kaplan survival curve of mice with chronic GVHD showed that the CTLA4-Ig and TJU103+CTLA4-Ig reduced the incidence of chronic GVHD, the TJU103 could delay the occurrence of chronic GVHD, but all the interference factors could not change the severity of chronic GVHD.
CONCLUSIONTJU103 can delay the onset time of chronic GVHD, and the CTLA4-Ig can reduce the incidences of cGVHD, the combining use of TJU103 and CTLA4-Ig can significantly reduce the incidence of chronic GVHD, but can not change the severity of chronic GVHD.
