Separation and simultaneous quantitation of PGF2 and its epimer 8--PGF2 using modifier-assisted differential mobility spectrometry tandem mass spectrometry.
10.1016/j.apsb.2018.01.011
- Author:
Chunsu LIANG
1
;
Hui SUN
1
;
Xiangjun MENG
1
;
Lei YIN
1
;
J Paul FAWCETT
2
;
Huaidong YU
3
;
Ting LIU
3
;
Jingkai GU
1
Author Information
1. Research Center for Drug Metabolism, School of Life Sciences, Jilin University, 2699 Qianjin Street, Changchun 130012, China.
2. School of Pharmacy, University of Otago, Dunedin, New Zealand.
3. Shanghai AB Sciex Analytical Instrument Trading Co., Ltd., Beijing 100015, China.
- Publication Type:Journal Article
- Keywords:
8-iso-PGF2α;
Differential mobility spectrometry;
Epimer;
Mass spectrometry;
PGF2α
- From:
Acta Pharmaceutica Sinica B
2018;8(2):228-234
- CountryChina
- Language:English
-
Abstract:
Because many therapeutic agents are contaminated by epimeric impurities or form epimers as a result of metabolism, analytical tools capable of determining epimers are increasingly in demand. This article is a proof-of-principle report of a novel DMS-MS/MS method to separate and simultaneously quantify epimers, taking PGF2 and its 8-epimer, 8--PGF2, as an example. Good accuracy and precision were achieved in the range of 10-500 ng/mL with a run time of only 1.5 min. Isopropanol as organic modifier facilitated a good combination of sensitivity and separation. The method is the first example of the quantitation of epimers without chromatographic separation.
