Correlation analysis between the chemical contents and bioactivity for the quality control of Alismatis Rhizoma.

Author: Xiaoxv GAO ¹ ; Chengpeng SUN ¹ ; Zhenglong YU ¹ ; Jian CANG ¹ ; Xiangge TIAN ¹ ; Xiaokui HUO ¹ ; Lei FENG ¹ ; Xinguang LIU ¹ ; Chao WANG ¹ ; Baojing ZHANG ¹ ; Xiaochi MA ¹
Author Information

1. College of Pharmacy, Academy of Integrative Medicine, Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China.
Publication Type:Journal Article
Keywords: Alismatis Rhizoma; Lipase; Qualitative analysis; Saccharide; Terpene
From: Acta Pharmaceutica Sinica B 2018;8(2):242-251
CountryChina
Language:English
Abstract: In order to clarify regions of production and to discriminate processing methods, quantitative and qualitative analyses for saccharides and terpenes in 35 batches of Alismatis Rhizoma were performed. Methodologies included HPLC-PDA, HPLC-VWD and UHPLC-MS , combined with principal component analysis (PCA) and partial least squares regression techniques (PLSR). The inhibitory effects of triterpenes and Alismatis Rhizoma extracts on lipase activity were evaluated . PLSR analysis revealed significant positive correlations ( = 0.5795) between the contents of triterpenes , , , and and the inhibitory effects of Alismatis Rhizoma. The present study establishes an effective method for simultaneous determination of multiple components, and identifies key bioactive triterpenes. These results can be used for systematic and novel analytical strategies for the quality control of Alismatis Rhizoma production.