Integrated metabolomic and transcriptomic analyses revealed the distribution of saponins in .
10.1016/j.apsb.2017.12.010
- Author:
Guangfei WEI
1
;
Linlin DONG
1
;
Juan YANG
1
;
Lianjuan ZHANG
1
;
Jiang XU
1
;
Feng YANG
1
;
Ruiyang CHENG
1
;
Ran XU
1
;
Shilin CHEN
1
Author Information
1. Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
AACT, acetoacetyl-CoA acyltransferase;
DS, dammarenediol-II synthase;
DXPR, 1-deoxy-d-xylulose 5-phosphate reductoisomerase;
DXPS, 1-deoxy-d-xylulose 5-phosphate synthase;
FPP, farnesyl diphosphate;
FPS, farnesyl pyrophosphate synthase;
GDPS, gerenyl diphosphatesynthase;
Gene expression;
Growth years;
HDS, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase;
HMGR, 3-hydroxy-3-methylglutaryl-CoA reductase;
HMGS, 3-hydroxy-3-methylglutaryl-CoA synthase;
HPLC—UV, high-performance liquid chromatography-ultraviolet detection;
IPP, isoprenyl diphosphate;
IPPI, isopentenyl pyrophosphate isomerase;
ISPD, 2-C-methylerythritol 4-phosphatecytidyl transferase;
ISPE, 4-(cytidine-5′-diphospho)-2-C-methylerythritol kinase;
ISPH, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase;
MECPS, 2-C-methylerythritol-2,4-cyclophosphate synthase;
MEP, 2-C-methyl-d-erythritol-4-phosphate;
MVA, mevalonate;
MVDD, mevalonate diphosphate decarboxylase;
MVK, mevalonate kinase;
Metabolomic analyses;
OPLS-DA, orthogonal partial least-squares discrimination analysis;
P450, P450-monooxygenase;
PCA, principal component analysis;
PDS, 20(S)-protopanaxadiol saponins;
PMK, phosphomevalonate kinase;
PTS, 20(S)-protopanaxatriol saponins;
Panax notoginseng;
SE, squalene epoxidase;
SS, squalene synthase;
Saponin;
UGTs, UDP-glycosyltransferases;
UPLC—MS, ultrahigh-performance liquid chromatography–mass spectrometry;
VIP, variable importance in projection
- From:
Acta Pharmaceutica Sinica B
2018;8(3):458-465
- CountryChina
- Language:English
-
Abstract:
is famous for its important therapeutic effects. Saponins are bioactive compounds found in different parts and developmental stages of plants. Thus, it is urgently to study saponins distribution in different parts and growth ages of plants. In this study, potential biomarkers were found, and their chemical characteristic differences were revealed through metabolomic analysis. High-performance liquid chromatography data indicated the higher content of saponins (, Rg1, Re, Rd, and Rb1) in the underground parts than that in the aerial parts. 20()-Protopanaxadiol saponins were mainly distributed in the aerial parts. Additionally, the total saponin content in the 3-year-old plant (188.0 mg/g) was 1.4-fold higher than that in 2-year-old plant (130.5 mg/g). The transcriptomic analysis indicated the tissue-specific transcription expression of genes, namely, , , , , and , which encoded critical synthases in saponin biosyntheses. These genes showed similar expression patterns among the parts of plants. The expression levels of these genes in the flowers and leaves were 5.2fold higher than that in the roots and fibrils. These results suggested that saponins might be actively synthesized in the aerial parts and transformed to the underground parts. This study provides insights into the chemical and genetic characteristics of to facilitate the synthesis of its secondary metabolites and a scientific basis for appropriate collection and rational use of this plant.
