Activation of an unconventional meroterpenoid gene cluster in leads to the production of new berkeleyacetals.
10.1016/j.apsb.2017.12.005
- Author:
Tao ZHANG
1
;
Jun WAN
1
;
Zhajun ZHAN
2
;
Jian BAI
1
;
Bingyu LIU
1
;
Youcai HU
1
Author Information
1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
2. College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China.
- Publication Type:Journal Article
- Keywords:
Berkeleyacetals;
Biosynthesis;
Cryptic gene cluster;
Genome mining;
Meroterpenoid;
Neosartorya glabra
- From:
Acta Pharmaceutica Sinica B
2018;8(3):478-487
- CountryChina
- Language:English
-
Abstract:
Fungal genomes carry many gene clusters seemingly capable of natural products biosynthesis, yet most clusters remain cryptic or down-regulated. Genome mining revealed an unconventional paraherquonin-like meroterpenoid biosynthetic gene cluster in the chromosome of . The cryptic or down-regulated pathway was activated by constitutive expression of pathway-specific regulator gene encoded within biosynthetic gene cluster. Chemical analysis of mutant -OE: extracts enabled the isolation of four berkeleyacetal congeners, in which two of them are new. On the basis of careful bioinformatic analysis of the coding enzymes in the gene cluster, the biosynthetic pathway of berkeleyacetals was proposed. These results indicate that this approach would be valuable for discovery of novel natural products and will accelerate the exploitation of prodigious natural products in filamentous fungi.
