Gold nanorods together with HSP inhibitor-VER-155008 micelles for colon cancer mild-temperature photothermal therapy.
10.1016/j.apsb.2018.05.011
- Author:
Xichuan TANG
1
;
Liwei TAN
2
;
Kun SHI
1
;
Jinrong PENG
1
;
Yao XIAO
1
;
Wenting LI
3
;
Lijuan CHEN
1
;
Qian YANG
4
;
Zhiyong QIAN
1
Author Information
1. State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, and Collaborative Innovation Center, Chengdu 610041, China.
2. College of Medicine, Southwest Jiaotong University, Chengdu 610031, China.
3. Department of Pharmacy, West China Second University Hospital, Sichuan University, Chengdu 610041, China.
4. School of Pharmacy, Key College Laboratory of Sichuan Province for Specific Structure of Small Molecule Drugs, Chengdu Medical College, Chengdu 610500, China.
- Publication Type:Journal Article
- Keywords:
Gold nanorods;
Micelle;
Phototherapy;
Tumor inhibitor;
VER-155008
- From:
Acta Pharmaceutica Sinica B
2018;8(4):587-601
- CountryChina
- Language:English
-
Abstract:
Enhancing the heat-sensitivity of tumor cells provides an alternative solution to maintaining the therapeutic outcome of photothermal therapy (PTT). In this study, we constructed a therapeutic system, which was composed of methoxy-polyethylene-glycol-coated-gold-nanorods (MPEG-AuNR) and VER-155008-micelles, to evaluate the effect of VER-155008 on the sensitivity of tumor cells to heat, and further investigate the therapeutic outcome of MPEG-AuNR mediated PTT combined with VER-155008- micelles. VER-155008- micelles down-regulate the expression of heat shock proteins and attenuate the heat-resistance of tumor cell. The survival of HCT116 cells treated with VER-155008- micelles under 45 °C is equal to that treated with high temperature hyperthermia (55 °C) . Furthermore, we proved either the MPEG-AuNR or VER-155008- micelles can be accumulate in the tumor site by photoacoustic imaging and fluorescent imaging. anti-cancer evaluation showed that tumor size remarkably decreased (smaller than 100 mm or vanished) when treated with combing 45 °C mild PTT system, which contrasted to the tumor size when treated with individual 45 °C mild PTT (around 500 nm) or normal saline as control (larger than 2000 nm). These results proved that the VER-155008- micelles can attenuate the heat-resistance of tumor cells and enhance the therapeutic outcome of mild-temperature photothermal therapy.
