Time-effect relationship and molecular mechanism of electroacupuncture for repair of gastric mucosal lesion.

Yuan Zhang; Caichun Liu; Linyu Lian; Jiacheng Shen; Qida HE; Jiaolong Chen; Fuqiang MA; Zongbao Yang

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Time-effect relationship and molecular mechanism of electroacupuncture for repair of gastric mucosal lesion.

Author: Yuan ZHANG ^{1
,

2
,

3} ; Caichun LIU ⁴ ; Linyu LIAN ⁵ ; Jiacheng SHEN ⁴ ; Qida HE ⁵ ; Jiaolong CHEN ⁵ ; Fuqiang MA ⁴ ; Zongbao YANG ⁴
Author Information

1. Research Institute of Shenzhen, Medical College of Xiamen University, Shenzhen 518000, Guangdong Province, China
2. College of Acupuncture-Moxibustion and Tuina, Hunan University of CM, Changsha 410208
3. 3Fujian University of TCM.
4. Research Institute of Shenzhen, Medical College of Xiamen University, Shenzhen 518000, Guangdong Province, China.
5. Fujian University of TCM.
Publication Type:Journal Article
Keywords: Point ST 21 (Liangmen); Point ST 36 (Zusanli); electroacupuncture (EA); gastric ulcer; neurotensin(NT); proliferating cell nuclear antigen (PCNA); substance P (SP); time-effect relationship
From: Chinese Acupuncture & Moxibustion 2018;38(7):747-752
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo dynamically observe the effects of electroacupuncture (EA) on repair of gastric mucosal lesion in rats with gastric ulcer, and to explore the time-effect relationship and molecular mechanism of EA for gastric ulcer.
METHODSA total of 72 SD rats were randomly assigned to a normal group, a model group, a acupoint group and a sham acupoint group, and each group were further divided into a 1-day subgroup, a 4-day subgroup and a 7-day subgroup, 6 rats in each subgroup. The rat model of gastric ulcer was established by using intragastric administration of ethyl alcohol. The rats in the acupoint group were treated with EA at"Zusanli"(ST 36) and"Liangmen"(ST 21); the rats in the sham acupoint group were treated with EA at points 5 mm next to"Zusanli"(ST 36) and"Liangmen"(ST 21); the EA was given 30 min per treatment, once a day. The rats in the normal group and model group were treated with immobilization for 30 min per day, and no EA was given. PR-PCR method was applied to test the expression of proliferating cell nuclear antigen (PCNA) and substance P (SP); Western blot method was applied to test the expression of neurotensin (NT).
RESULTSAfter 1-day treatment, the ulcer index in the model group was higher than that in the normal group (<0.01), and the expression of PCNA, SP and NT was decreased (<0.01, <0.05); compared with the model group and sham acupoint group, the ulcer index was decreased in the acupoint group (both <0.05), and the expression of PCNA and SP was up-regulated (all <0.05) while that of NT was up-regulated (both <0.01). After 4-day treatment, the ulcer index in the model group was reduced but still higher than that in the normal group (<0.05), and the expression of PCNA, SP and NT was up-regulated and higher than that in the normal group (all <0.01); the ulcer index in the acupoint group was similar to that in the normal group (>0.05), and the expression of PCNA and SP was lower than that in the model group (<0.01, <0.05), and the expression of NT was not significantly different from that in the model group (>0.05). After 7-day treatment, the differences of indexes above were not significant among the four groups (all >0.05).
CONCLUSIONEA at acupoints of stomach meridian has two-way regulation on PCNA and SP and improve the expression of NT in different pathological state of gastric ulcer, which could further improve the repair of gastric ulcer.