Identify metabolites of Piper longum alkaloids in feces and urine of rat by HPLC-FT-MS.
10.19540/j.cnki.cjcmm.20180305.003
- Author:
Rong-Rong XU
1
;
Hao-Long LIU
2
;
Xiao-Qing CHEN
1
;
Yin-Ying BA
1
;
Xing WANG
1
;
Xia WU
1
Author Information
1. Beijing Key Lab of Traditional Chinese Medicine, Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
2. Beijing Key Laboratory of Analysis and Evaluation on Chinese Medicine, Beijing Institute for Drug Control, Beijing 100035, China.
- Publication Type:Journal Article
- Keywords:
HPLC-FT-MS/MS;
Piper longum alkaloids;
feces;
metabolites;
urine
- From:
China Journal of Chinese Materia Medica
2018;43(9):1798-1805
- CountryChina
- Language:Chinese
-
Abstract:
The metabolites were detected in feces and urine of rats orally administrated alkaloids of Piper longum by using high performance liquid chromatography coupled with a Fourier Transform ion cyclotron resonance mass spectrometer (HPLC-FT-MS). According to the mass spectrometric data and reported literature, the structures of metabolites were identified. Several metabolites were analyzed and belonged to piperine, piperanine, piperlonguminine, Δα,β-dihydropiperlonguminine and pellitorine, respectively. The metabolites of alkaloids from P. longum alkaloids were produced through Ⅰ phase and Ⅱ phase metabolism reaction, and were excreted with urination and defecation. The approach provided a rapid method for characterizing the metabolites of P. longum alkaloids and gave the truly active structures and the action mechanism of their neuroprotective effects.
