Mechanisms of Hirudo in promoting blood circulation and removing stasis based on network pharmacology.
10.19540/j.cnki.cjcmm.2018.0065
- Author:
Luo-Dan OUYANG
1
;
Xiao-Song HU
1
;
Ming NIU
2
;
Wen GAO
3
;
Yu CHENG
4
;
Jia-Bo WANG
2
;
Li MA
1
Author Information
1. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
2. China Military Institute of Chinese Medicine, 302 Military Hospital, Beijing 100039, China.
3. Industrial Management Center, Capital Medical University, Beijing 100069, China.
4. Department of Dermatology, Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing 100010, China.
- Publication Type:Journal Article
- Keywords:
Hirudo;
mechanism;
network pharmacology;
promoting blood circulation and removing stasis
- From:
China Journal of Chinese Materia Medica
2018;43(9):1901-1906
- CountryChina
- Language:Chinese
-
Abstract:
The study aims to analyze the mechanisms of Hirudo in promoting blood circulation and removing blood stasis based on network pharmacology. A database of chemical components of Hirudo was established through literature retrieval. The targets were predicted by using the reverse pharmacophore matching method and screened according to the antithrombotic and anticoagulant drug targets approved by FDA in the DrugBank database. Then, the targets were analyzed by KEGG pathway analysis, the protein interactions were analyzed by using BioGrid database, and the active constituents-target-pathway network model of Hirudo was established to study the mechanisms of Hirudo in promoting blood circulation and removing blood stasis. This study collected 49 chemical components of Hirudo, including amino acid, polypeptide, fatty acid ester, alkaloid, glycosides, and steroid. Totally 376 targets were predicted, and 5 critical targets related to the effects of Hirudo in promoting blood circulation and removing blood stasis were screened, including fibrinogen gamma chain, plasminogen, prothrombin, Urokinase-type plasminogen activator and coagulation factor X. The potential regulatory pathways included complement and coagulation cascades, platelet activation, VEGF signaling pathway, focal adhesion. This study reflects the multi-component, multi-target and multi-pathway features of Hirudo, and provides a scientific basis for elucidating the mechanisms of action of Hirudo in promoting blood circulation and removing blood stasis, as well as a reference for the study of mechanisms of traditional Chinese medicine.
