miR-93-5p Transferred by Exosomes Promotes the Proliferation of Esophageal Cancer Cells via Intercellular Communication by Targeting PTEN.

Meng Xin Liu; Juan Liao; Ming Xie; Zhi Kui Gao; Xiang Hu Wang; Ying Zhang; Mu He Shang; Li Hong Yin; Yue Pu PU; Ran Liu

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miR-93-5p Transferred by Exosomes Promotes the Proliferation of Esophageal Cancer Cells via Intercellular Communication by Targeting PTEN.

Author: Meng Xin LIU ¹ ; Juan LIAO ^{2
,

3} ; Ming XIE ⁴ ; Zhi Kui GAO ¹ ; Xiang Hu WANG ¹ ; Ying ZHANG ¹ ; Mu He SHANG ¹ ; Li Hong YIN ¹ ; Yue Pu PU ¹ ; Ran LIU ¹
Author Information

1. Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, China.
2. Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, China
3. Hangzhou First People's Hospital, Hangzhou 310000, Zhejiang, China.
4. North China Petroleum Bureau General Hospital, Renqiu 062552, Hebei, China.
Publication Type:Journal Article
Keywords: Esophageal cancer; Exosomes; Plasma biomarker; miR-93-5p
MeSH: Aged; Cell Communication; China; Esophageal Neoplasms; physiopathology; Exosomes; physiology; Female; Humans; Male; MicroRNAs; metabolism; Middle Aged; PTEN Phosphohydrolase; genetics; metabolism; Risk
From: Biomedical and Environmental Sciences 2018;31(3):171-185
CountryChina
Language:English
Abstract:
OBJECTIVETo investigate the relationship between plasma miR-93-5p and the risk of esophageal cancer, as well as the influence of miR-93-5p on the biological function of esophageal cancer cells, exerted through exosomes.
METHODSThe expression of plasma miR-93-5p in esophageal cancer patients and healthy controls was analysed by real-time quantitative PCR. The influence of miR-93-5p on the risk and prognosis of esophageal carcinoma was analyzed by conditional logistic regression and survival analysis. The effect of miR-93-5p on the biological function of recipient cells was investigated by establishing an in vitro donor cell co-culture model. The target gene of miR-93-5p was validated by luciferase reporter assay and Western Blotting.
RESULTSUpregulation of plasma miR-93-5p expression significantly increases the risk of esophageal cancer and is associated with poor prognosis. miR-93-5p transferred by exosomes promotes the proliferation of recipient esophageal cancer cells and affects the expression of PTEN and its downstream proteins p21 and cyclin D1.
CONCLUSIONOur study provides a reference for the identification of biomarkers for the diagnosis and prognosis of esophageal cancer.