Molecular Characterization and Correlation with β-lactam Resistance of Streptococcus pneumonia Isolates in Hangzhou, China.

Mei Fen Chu; Xiao Xiang Liu; Shao Ni Zhang; Yan Ying Huang; Peng DU; Li Fang Wang; Lei JI; Jie Yan; Ai Hua Sun

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Molecular Characterization and Correlation with β-lactam Resistance of Streptococcus pneumonia Isolates in Hangzhou, China.

Author: Mei Fen CHU ¹ ; Xiao Xiang LIU ² ; Shao Ni ZHANG ^{3
,

4} ; Yan Ying HUANG ^{3
,

4} ; Peng DU ² ; Li Fang WANG ² ; Lei JI ² ; Jie YAN ^{5
,

6} ; Ai Hua SUN ²
Author Information

1. Department of Laboratory Medicine, Hangzhou Medical College, Hangzhou 310053, Zhejiang, China.
2. Faculty of Basic Medicine, Hangzhou Medical College, Hangzhou 310053, Zhejiang, China.
3. Faculty of Basic Medicine, Hangzhou Medical College, Hangzhou 310053, Zhejiang, China
4. School of Laboratory Medical Science and Life Science, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China.
5. Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital of Zhejiang University, Hangzhou 310003, Zhejiang, China
6. Department of Medical Microbiology and Parasitology, College of Medicine, Zhejiang University, Hangzhou 310058, Zhejiang, China.
Publication Type:Journal Article
MeSH: Anti-Bacterial Agents; pharmacology; China; epidemiology; Drug Resistance, Bacterial; Humans; Pneumococcal Infections; epidemiology; microbiology; Streptococcus pneumoniae; drug effects; genetics; beta-Lactams; pharmacology
From: Biomedical and Environmental Sciences 2018;31(5):389-393
CountryChina
Language:English
Abstract: Penicillin-binding proteins (PBPs) are the target of β-lactam antibiotics (the major treatment for Streptococcus pneumoniae infections), and mutations in PBPs are considered as a primary mechanism for the development of β-lactam resistance in S. pneumoniae. This study was conducted to investigate the mutations in the PBPs of clinical S. pneumoniae isolates in Hangzhou, China, in correlation with β-lactam resistance. Results showed that 19F was the predominant serotype (7/27) and 14 of the S. pneumoniae isolates were resistant to both penicillin G and cephalosporin. Genotyping results suggested that β-lactam-resistant isolates primarily exhibited single-site mutations in both the STMK and SRNVP motifs of pbp1a in combination with double-site mutations in the STMK motif of pbp2x, which might be the primary mechanisms underlying the β-lactam resistance of the isolates in this study.