Overexpression of YOD1 Promotes the Migration of Human Oral Keratinocytes by Enhancing TGF-β3 Signaling.
- Author:
Qiang JU
1
;
Meng Xue LI
1
;
Gang CHEN
1
;
Heng Xue WANG
1
;
Qiao Mei SHI
1
;
Xing GE
1
;
Zhen DING
1
;
Qi WANG
1
;
Li Chun XU
1
Author Information
- Publication Type:Journal Article
- Keywords: Cell migration; De-ubiquitinating enzyme YOD1; Non-syndromic cleft lip and palate; Smads; Transforming growth factor-β
- MeSH: Cell Movement; physiology; Cell Proliferation; Cells, Cultured; Endopeptidases; genetics; metabolism; Humans; Keratinocytes; physiology; Signal Transduction; physiology; Smad Proteins; genetics; metabolism; Thiolester Hydrolases; genetics; metabolism; Transforming Growth Factor beta3; genetics; metabolism
- From: Biomedical and Environmental Sciences 2018;31(7):499-506
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the effects of YOD1 overexpression on the proliferation and migration of human oral keratinocytes (HOKs), and to clarify whether the mechanisms involve transforming growth factor-β (TGF-β) signaling.
METHODSHOKs were transfected with the plasmid pEGFP-N3-YOD1 containing YOD1. The mRNA levels of YOD1 and TGF-β were determined by qPCR. The protein expressions of YOD1, TGF-β, Smad2/3, Smad4, and phospho-Smad2/3 were determined by western blotting. Cell proliferation and migration were evaluated by Cell Counting Kit-8 assay and wound healing assay, respectively.
RESULTSThe mRNA and protein levels of YOD1 were higher in HOKs transfected with YOD1. YOD1 overexpression significantly enhanced the migration of HOKs. The mRNA and protein levels of TGF-β3 were increased by YOD1 overexpression. HOKs transfected with YOD1 exhibited increased phospho-Smad2/3 levels.
CONCLUSIONYOD1 overexpression enhances cell migration by promoting TGF-β3 signaling which may play an important role in lip and palate formation. YOD1 mutation may contribute to aberrant TGF-β3 signaling associated with decreased cell migration resulting in NSCLP.
