Expression of Pref-1 and Related Chemokines during theDevelopment of Rat Mesenteric Lymph Nodes.
- Author:
Yan PENG
1
;
Li Min JIA
1
;
Bao Xin LI
2
;
Li Ping XIE
1
;
Zun Jiang XIE
1
;
Jin Hua ZHENG
1
Author Information
- Publication Type:Journal Article
- Keywords: Adipocyte progenitor cells; Chemokines; Development; Mesenteric lymph nodes; Rat; Ultrastructure
- MeSH: Animals; Chemokines; genetics; metabolism; Female; Gene Expression Regulation, Developmental; physiology; Intercellular Signaling Peptides and Proteins; genetics; metabolism; Lymph Nodes; embryology; metabolism; Membrane Proteins; genetics; metabolism; Mesentery; embryology; Pregnancy; Rats
- From: Biomedical and Environmental Sciences 2018;31(7):507-514
- CountryChina
- Language:English
-
Abstract:
OBJECTIVEThe aim of this study was to investigate the ability of Pref-1+ adipocyte progenitor cells to mobilize into mesenteric lymph nodes (MLNs) and the dynamic expression of related chemokines during the development of rat MLNs.
METHODSImmunohistochemical analyses were used to detect the expression of Pref-1 and related chemokines. Transmission electron microscopy (TEM) was used to observe the changes in ultrastructure of MLNs.
RESULTSCells containing lipid droplets were found in all rat MLNs at embryonic day (E) 18.5, 2 and 6 weeks (w) after birth, and they were similar to fibroblastic reticular cells (FRCs) or follicular dendritic cells (FDCs) under TEM. Pref-1+ adipocyte progenitor cells were found in all MLNs. The expression level of Pref-1 was significantly increased at 2 w after birth and decreased at 6 w after birth. The tendency of Cxcl12 expression was consistent with that of Pref-1 and was positively correlated with the expression of Pref-1 (P < 0.01; r = 0.897). At E18.5, Cxcl13, and Ccr7 were significantly expressed in the MLN anlage, but the expression level of Ccl21 was low. The expression level of Cxcl13, Ccr7, and Ccl21 in MLN were significantly increased at 2 w after birth (P < 0.05), while the expression of Ccr7 and Ccl21 were significantly decreased at 6 w after birth (P < 0.05).
CONCLUSIONAdipocyte progenitor cells are involved in the rat MLNs development through differentiation into FRC and FDC. The expression of the relevant chemokines during the development of MLNs is dynamic and may be related to the maintenance of lymph nodes self-balance state.
