Evaluation of the New Automatic Mycob.T Stainer and Scanner for Detecting Acid-fast Bacilli in China.

Qiang JU; Meng Xue LI; Gang Chen; Heng Xue Wang; Qiao Mei Shi; Xing GE; Zhen Ding; Qi Wang; Li Chun XU

Return

Evaluation of the New Automatic Mycob.T Stainer and Scanner for Detecting Acid-fast Bacilli in China.

Author: Qiang JU ¹ ; Meng Xue LI ² ; Gang CHEN ³ ; Heng Xue WANG ⁴ ; Qiao Mei SHI ³ ; Xing GE ⁵ ; Zhen DING ² ; Qi WANG ² ; Li Chun XU ¹
Author Information

1. Beijing Physical Examination Center, Beijing 100077, China.
2. National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
3. Anhui Provincial Chest Hospital, Hefei 230022, Anhui, China.
4. Shandong Provincial Chest Hospital, Jinan 250013, Shandong, China.
5. Linyi People's Hospital, Linyi 276003, Shandong, China.
Publication Type:Journal Article
Keywords: Automatic; Scanner; Stainer; Tuberculosis
From: Biomedical and Environmental Sciences 2018;31(8):572-578
CountryChina
Language:English
Abstract:
OBJECTIVETo validate the performance of Mycob. T Stainer and Scanner (MTSS) for detecting acid-fast bacilli (AFB).
METHODSA total of 3,816 sputum samples from 1,515 tuberculosis (TB) suspects were tested at the Anhui Provincial Chest Hospital and the Linyi People's Hospital from April-August, 2016. Each specimen was placed on two smear slides. One slide was stained by the ziehl-neelsen (ZN) method to be read by conventional microscopy (CM). The other slide was stained and scanned by MTSS. All specimens were decontaminated with 4% NaOH, and then inoculated into solid culture. The performance of MTSS was assessed.
RESULTSMTSS produced higher average positivity rate (27.96%) as compared with the CM (26.83%). The overall sensitivity and specificity of MTSS were 78.9% and 93.9%, respectively. The sensitivity and specificity of CM was 77.4% and 95.0%, respectively.
CONCLUSIONMTSS exhibited a favorable performance in the detection of AFB. It may be an alternative to CM for screening TB.