Variant of Associated with Increasing Risk in Chinese Patients with Relapsing-remitting Multiple Sclerosis.
- Author:
Sheng CHEN
1
;
Juan ZHANG
2
;
Qi-Bing LIU
3
;
Jing-Cong ZHUANG
3
;
Lei WU
2
;
Yong-Feng XU
2
;
Hong-Fu LI
2
;
Zhi-Ying WU
2
;
Bao-Gou XIAO
1
Author Information
- Publication Type:Journal Article
- Keywords: Genetic Association Studies; Multiple Sclerosis; Risk Factors; Single-nucleotide Polymorphism
- MeSH: Adolescent; Adult; Aged; Alleles; Asian Continental Ancestry Group; Female; Gene Frequency; genetics; Genetic Predisposition to Disease; genetics; Genome-Wide Association Study; Genotype; Humans; Male; Middle Aged; Multiple Sclerosis; genetics; Odds Ratio; Polymorphism, Single Nucleotide; genetics; T-Box Domain Proteins; genetics; Young Adult
- From: Chinese Medical Journal 2018;131(6):643-647
- CountryChina
- Language:English
-
Abstract:
BackgroundMultiple sclerosis (MS) is a common central nervous system autoimmune disorder. Increasing number of genome-wide association study (GWAS) analyses hint that MS is strongly associated with genetics. Unfortunately, almost all the GWAS analyses were Caucasian population based. Numbers of risk loci might not be replicated in Chinese MS patients. Hence, we performed a MassArray Assay to genotype the previously reported variants located in the transcription regulation genes in order to elucidate their role in the Chinese MS patients.
MethodsOne hundred and forty-two relapsing-remitting MS (RRMS) patients and 301 healthy controls were consecutively collected from September 2, 2008, to June 7, 2013, as stage 1 subjects. Eight reported transcription regulation-related single-nucleotide polymorphisms (SNPs) were genotyped using the Sequenom MassArray system. In stage 2, another 44 RRMS patients and 200 healthy controls were consecutively collected and Sanger sequenced from April 7, 2015, to June 29, 2017, for the validation of positive results in stage 1. Differences in allele and genotype frequencies between patients and healthy controls, odds ratios, and 95% confidence intervals were calculated with the Chi-square test or Fisher's exact test. Hardy-Weinberg equilibrium was tested also using the Chi-square test.
ResultsIn stage 1 analysis, we confirmed only one previously reported risk variant, rs11129295 in EOMES gene. We found that the frequency of T/T genotype was much higher in MS group (χ = 10.251, P = 0.005) and the T allele of rs11129295 increased the risk of MS (χ = 10.022, P = 0.002). In stage 2 and combined analyses, the T allele of rs11129295 still increased the risk of MS (χ = 4.586, P = 0.030 and χ = 16.378, P = 5.19 × 10, respectively).
ConclusionsThis study enhances the knowledge that the variant of EOMES is associated with increasing risk in Chinese RRMS patients and provides a potential therapeutic target in RRMS.