Quality evaluation of medicinal herb decoction--Forsythiae Fructus.
10.19540/j.cnki.cjcmm.20171023.008
- Author:
Jiao XU
1
;
Jung CHAO
1
;
Yun-Tao DAI
1
;
Chao ZHU
2
;
Qi LI
3
;
Tomoda TAKEHISA
4
;
Dan-Dan WANG
5
;
Shi-Lin CHEN
1
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medicinal Sciences, Beijing 100700, China.
2. College of Medicine and Nursing, Dezhou University, Dezhou 253023, China.
3. Shanghai Traditional Chinese Medicine Co., Ltd., Shanghai 200002, China.
4. Tsumura & Co., Tokyo 107-8521, Japan.
5. Waters Corporation (Shanghai), Shanghai 201206, China.
- Publication Type:Journal Article
- Keywords:
Forsythiae Fructus;
extraction ratio;
fingerprint;
mass spectrometry;
quality evaluation;
standard decoction of single medicinal herb;
transfer rate
- From:
China Journal of Chinese Materia Medica
2018;43(5):868-872
- CountryChina
- Language:Chinese
-
Abstract:
To establish the quality control methods for the standard decoction of Forsythiae Fructus. Twelve batches of representative Forsythiae Fructus were collected to prepare standard decoction of Forsythiae Fructus, and then the parameters such as extraction ratio, transfer rate of the index components and pH value of the solution were calculated to evaluate the stability of the process. The simultaneous determination method of target components and fingerprint method were established, and ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was used to identify the main common peaks in the fingerprint to clarify the main chemical constituents in the decoction. The similarities of the fingerprints of standard decoction of Forsythiae Fructus were more than 0.9. The average extraction ratio of the standard decoction of Forsythiae Fructus was (15.53±6.27)%, and the transfer rate of forsythiaside A was(38.0±10.2)%. The method for evaluating the quality of standard decoction of Forsythiae Fructus was presented, providing reference for the quality control of products stemmed from the water extract of Forsythiae Fructus, with high similarity and uniform quality.
