Molecular cloning and characterization of CMK from Artemisia annua.
10.19540/j.cnki.cjcmm.20180329.007
- Author:
Yu-Fang FAN
1
;
Man ZHANG
1
;
Li-En XIANG
1
;
Fang-Yuan ZHANG
1
;
Xiao-Zhong LAN
2
;
Zhi-Hua LIAO
1
;
Chun-Xian YANG
1
Author Information
1. Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Life Science, Southwest University, Chongqing 400715, China.
2. TAAHC-SWU Medicinal Plant Joint R & D Centre, Xizang Agricultural and Husbandry College, Nyingchi 860000, China.
- Publication Type:Journal Article
- Keywords:
Artemisia annua;
CMK;
MEP;
artemisinin
- From:
China Journal of Chinese Materia Medica
2018;43(11):2264-2260
- CountryChina
- Language:Chinese
-
Abstract:
Artemisinin is a preferred medicine in the treatment of malaria. In this study, AaCMK, a key gene involved in the upstream pathway of artemisinin biosynthesis, was cloned and characterized from Artemisia annua for the first time. The full-length cDNA of AaCMK was 1 462 bp and contained an ORF of 1 197 bp that encoded a 399-anomo-acid polypeptide. Tissue expression pattern analysis showed that AaCMK was expressed in leaves, flowers, roots and stems, but with higher expression level in glandular secretory trichomes. In addition, the expression of AaCMK was markedly increased after MeJA treatment. Subcellular localization showed that the protein encoded by AaCMK was localized in chloroplast. Overexpression of AaCMK in Arabidopsis increased the contents of chlorophyll a, chlorophyll b and carotenoids. These results suggest that AaCMK plays an important role in the biosynthesis of terpenoids in A. annua and this research provids a candidate gene that could be used for engineering the artemisinin biosynthesis.
