Expression of DKKL1 in spermatozoa of men with asthenospermia.

Qiu-xia Yan; Yi MA; Run-qiang Chen; Xiu-qin Zhou; Jing Qiao; Ying-jie Xian; Ling Feng; Cai-rong Chen

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Expression of DKKL1 in spermatozoa of men with asthenospermia.

Author: Qiu-Xia YAN ^{1
,

2} ; Yi MA ; Run-Qiang CHEN ; Xiu-Qin ZHOU ; Jing QIAO ; Ying-Jie XIAN ; Ling FENG ; Cai-Rong CHEN
Author Information

1. 1Center for Reproductive Medicine, Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, Qingyuan 511518,China
2. 2Department of Cellular Biology, Institute of Biological Medicine, Jinan University, Guangzhou 510632, China. E-mail: yanqiuxia1982@163.com.
Publication Type:Journal Article
From: Journal of Southern Medical University 2018;38(3):324-328
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo compare the expression of DKKL1 in ejaculated spermatozoa of normal fertile men and men with asthenospermia and investigate the role of DKKL1 in the pathogenesis of asthenospermia.
METHODSThe characteristics of semen samples collected from normal fertile men and men with asthenospermia were analyzed using computer-assisted sperm analysis according to WHO criteria. The ejaculated sperms were isolated by Percoll discontinuous density gradients to detect the expression of DKKL1 mRNA and protein using real-time PCR and Western blotting.
RESULTSThe expression of DKKL1 mRNA was significantly down-regulated by 11.1 times in asthenospermic men as compared with that in normal fertile men (P<0.01). Western blotting showed that the expression of DKKL1 protein was down-regulated by 2.4 times in asthenospermic men compared to normal fertile men.
CONCLUSIONThe expression of DKKL1, which may play an important role in sperm motility,is significantly decreased in ejaculated spermatozoa of men with asthenospermia.