Quality control of Dandeng Tongnao Ruanjiaonang based on UPLC fingerprint combined with chemical pattern recognition.
10.19540/j.cnki.cjcmm.20180611.006
- Author:
Lin ZHOU
1
;
Zhi SUN
1
;
Wen-Hua XUE
1
;
Shu-Hong LIANG
1
;
Li-Hua ZUO
1
;
Juan DING
2
;
Ling-Ling ZHAO
3
;
Xiao-Fang JIANG
3
;
Qing-Quan JIA
1
;
Hua WEI
4
;
Ke-Feng LIU
1
;
You-Hong HU
2
;
Jie ZHAO
1
Author Information
1. Department of Pharmacy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
2. Department of Quality Control, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
3. Department of Chinese Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
4. Medical College, Henan Polytechnic University, Jiaozuo 454000, China.
- Publication Type:Journal Article
- Keywords:
Dandeng Tongnao Ruanjiaonang;
chemical pattern recognition;
fingerprint;
quality control
- From:
China Journal of Chinese Materia Medica
2018;43(16):3279-3284
- CountryChina
- Language:Chinese
-
Abstract:
To establish the ultra performance liquid chromatography (UPLC) fingerprint of Dandeng Tongnao Ruanjiaonang and conduct a systemic, comprehensive quality evaluation of the drug by combining with a chemical pattern recognition method. In this study, Waters UPLC ultra-high performance liquid chromatography instrument and ACQUITY UPLCHSS T3 chromatographic colum n were employed to perform the separation with acetonitrile-0.1% formic acid aqueous solution as the mobile phase for gradient elution; and the detection wavelength was set at 256 nm to establish the UPLC fingerprint of 10 batches of Dandeng Tongnao Ruanjiaonang. Then, the further quality assessment of the drug was carried out by similarity evaluation, Cluster Analysis(CA), Principal Component Analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). Finally, 77 peaks were recognised as common peaks in the fingerprint, and 15 peaks of them were identified using standard references. The similarity value of these 10 batches of drugs was all above 0.960, indicating a relatively stable quality. But minor differences were still discovered between the batches of the drug by CA and PCA. Finally, 6 common peaks were recognised as the quality makers using OPLS-DA method. The analysis method established in this study was scientific, accurate, reliable and simple; fingerprint combined with chemical pattern recognition technique can be used to systematically and comprehensively evaluate the drug quality of Dandeng Tongnao Ruanjiaonang; what's more, it could also provide a reference for the quality control of traditional Chinese medicine and its preparations at the same time.
