The change of expression of interleukin-6 and -8 after the application of the static compressive pressure on the fibroblast originated from the periodontal ligaments.
- Author:
Yeon Hee LEE
1
;
Seong Gon KIM
;
Dong Seok NAHM
Author Information
1. Department of Orthodontics, College of Dentistry, Seoul National University, Korea.
- Publication Type:Original Article
- Keywords:
IL-6;
IL-8;
Compressive force
- MeSH:
Bone Resorption;
Enzyme-Linked Immunosorbent Assay;
Fibroblasts*;
Interleukin-6*;
Interleukin-8;
Periodontal Ligament*;
RNA, Messenger;
Tooth
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2006;32(5):426-429
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The fibroblast in the periodontal ligaments received various stress. Among them, compression and tension are quite important and they are related to the remodeling of tooth and alveolar bone. We studied the change of expression of interleukin-6 (IL-6) and interleukin-8 (IL-8) in the fibroblasts of the periodontal ligaments by real-time RT-PCR and ELISA. In results, the relative activity of IL-6 mRNA in 2 hours after was 1.54+/-0.08 and 1.00+/-0.05 in control and test, respectively (P<0.05). Its 12 hours after was 1.23+/-0.06 and 2.78+/-0.14 in control and test, respectively (P<0.05). The relative activity of IL-8 mRNA in 2 hours after was 1.00+/-0.05 and 0.24+/-0.01 in control and test, respectively (P<0.05). Its 12 hours after was 1.23+/-0.06 and 0.63+/-0.03 in control and test, respectively (P<0.05). The concentration of IL-6 was 1.02+/-0.16 ng/ml, 0.90+/-0.14 ng/ml, and 1.32+/-0.12 ng/ml (P<0.05) in control, 2, and 12 hours after, respectively. The concentration of IL-8 was 2.26+/-0.17 ng/ml, 1.70+/-0.26 ng/ml (P<0.05), and 0.84+/-0.47 ng/ml (P<0.05) in control, 2, and 12 hours after, respectively. In conclusion, the expression of IL-6 was significantly increased after the application of the static compressive force, but IL-8 was significantly decreased. Considering their known function, their expression is quite important in tooth and bone resorption.
