Detection of procalcitonin based on fluorescence immune chromatography.
- Author:
Chuanzhi LIU
1
;
Wei LI
1
;
Guiying LIU
1
;
Yu YANG
1
;
Ping GONG
1
;
Yue HOU
1
Author Information
- Publication Type:Journal Article
- Keywords: fluorescence immune chromatography; procalcitonin; quantum dot; test strip
- From: Chinese Journal of Biotechnology 2018;34(3):440-448
- CountryChina
- Language:Chinese
- Abstract: Procalcitonin (PCT) is the precursor of calcitonin related to the severity of human bacterial infection. We made a test strip by coupling anti-PCT to quantum dot, in order to develop a highly sensitive and convenient PCT testing product. The anti-PCT titer had reached 10⁷ because of the stability by coupling anti-PCT with quantum dot. The detecting linear range of the experiment was 0.15 to 120 μg/L, the sensitivity was 0.007 μg/L, the recovery range was 91% to 113%, and the intra- and inter-assay coefficient of variation was less than 8%. Comparing the homemade fluorescence-detected test strip with PCT ELISA kit on sale, we got accurate results which could mostly accomplish the test of clinical samples.
