Progress in the spectral library based protein identification strategy.

Derui YU; Jie MA; Zengyan Xie; Mingze Bai; Yunping Zhu; Kunxian Shu

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Progress in the spectral library based protein identification strategy.

Author: Derui YU ¹ ; Jie MA ² ; Zengyan XIE ¹ ; Mingze BAI ¹ ; Yunping ZHU ² ; Kunxian SHU ¹
Author Information

1. Institute of Bioinformatics, Chongqing University of Posts and Telecommunications, Chongqing 400065, China.
2. National Center for Protein Sciences, State Key Laboratory of Proteomics, Beijing Proteome Research Center, Engineering Research Center for Protein Drugs, Institute of Radiation Medicine, Beijing 102206, China.
Publication Type:Journal Article
Keywords: protein identification; spectral libraries; spectral library searching; spectrum clustering; tandem mass spectrometry
From: Chinese Journal of Biotechnology 2018;34(4):525-536
CountryChina
Language:Chinese
Abstract: Exponential growth of the mass spectrometry (MS) data is exhibited when the mass spectrometry-based proteomics has been developing rapidly. It is a great challenge to develop some quick, accurate and repeatable methods to identify peptides and proteins. Nowadays, the spectral library searching has become a mature strategy for tandem mass spectra based proteins identification in proteomics, which searches the experiment spectra against a collection of confidently identified MS/MS spectra that have been observed previously, and fully utilizes the abundance in the spectrum, peaks from non-canonical fragment ions, and other features. This review provides an overview of the implement of spectral library search strategy, and two key steps, spectral library construction and spectral library searching comprehensively, and discusses the progress and challenge of the library search strategy.