Comparison of urinary proteomes among rats, guinea pigs and golden hamsters.

Na NI; Jianqiang WU; Li LI; Youhe Gao

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Comparison of urinary proteomes among rats, guinea pigs and golden hamsters.

Author: Na NI ¹ ; Jianqiang WU ² ; Li LI ¹ ; Youhe GAO ³
Author Information

1. Institute of Molecular Medicine and Cancel, Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China.
2. Department of Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100005, China.
3. Gene Engineering and Biotechnology Beijing Key Laboratory, Department of Biochemistry and Molecular Biology, Beijing Normal University, Beijing 100875, China.
Publication Type:Journal Article
Keywords: LC-MS/MS; animal model; golden hamster; guinea pig; rat; urine proteomics
From: Chinese Journal of Biotechnology 2018;34(4):578-585
CountryChina
Language:Chinese
Abstract: Biomarkers are the detectable changes associated with physiological or pathological changes. Urine as excreta of the body, without the mechanisms to maintain a homeostatic internal environment, can reflect a variety of changes in the body. Using animal models can simulate human disease processes, monitor disease changes, and provide clues to early diagnosis. Rats as commonly used model animals are not the dominant models for all disease, thus comparing the urinary proteins of rats with other animals to provide clues to the selection of other animal dominant models. In this study, urinary proteins were digested and profiled by liquid chromatography and tandem mass spectrometry (LC-MS/MS). The urinary proteins of rats, guinea pigs and golden hamsters were compared. The results showed that the number of urine proteins in the three different animals was different, and also different in every system of the body. This provides a basis for selecting the best animal models for different diseases.