Identification of mouse brain neuropeptides by high throughput mass spectrometry.

Xianfeng Shao; Min MA; Ruibing Chen; Chenxi Jia

Return

Identification of mouse brain neuropeptides by high throughput mass spectrometry.

Author: Xianfeng SHAO ¹ ; Min MA ² ; Ruibing CHEN ¹ ; Chenxi JIA ²
Author Information

1. School of Basic Medicine, Tianjin Medical University, Tianjin 300070, China.
2. Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences-Beijing, Beijing 102206, China.
Publication Type:Journal Article
Keywords: LC-MS/MS; endogenous peptides; high-throughput detection; neuropeptides; whole brain tissue
From: Chinese Journal of Biotechnology 2018;34(4):594-601
CountryChina
Language:Chinese
Abstract: Neuropeptides play an important role in the physiological functions of the human body. The physiological activities such as pain, sleep, mood, learning and memory are affected by neuropeptides. Neuropeptides mainly exist in the nerve tissue of the body, and a small amount of them are distributed in body fluid and organs. At present, analysis of large-scale identification of neuropeptides in whole brain tissue is still challenging. Therefore, high-throughput detection of these neuropeptides is greatly significant to understand the composition and function of neuropeptides. In this study, 1 830 endogenous peptides and 99 novel putative neuropeptides were identified by extraction of endogenous peptides from whole brain tissue of mice by liquid phase tandem mass spectrometry (LC-MS / MS). The identification of these endogenous peptides provides not only a reference value in the treatment and mechanism studies of diseases and the development of drugs, but also the basis for the study of a new neuropeptides and their functions.