The Effects of Interleukin-17 on Production of Vascular Endothelial Growth Factor in Cultured Rheumatoid Arthritis Synoviocytes.
- Author:
Ihm Soo KWAK
1
;
Tae Soo NAM
;
Ha Yeon RHA
;
Jeung Tak SUH
;
Yoo Sun KIM
;
Sung Il KIM
Author Information
1. Department of Internal Medicine, College of Medicine, Pusan National University, Pusan, Korea. ksimd@hyowon.pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Interleukin-17;
Vascular endothelial growth factor;
Rheumatoid arthritis synoviocytes
- MeSH:
Arthritis, Rheumatoid*;
Enzyme-Linked Immunosorbent Assay;
Humans;
Interleukin-1;
Interleukin-17*;
Interleukin-1beta;
Necrosis;
Synovitis;
Transforming Growth Factor beta;
Tumor Necrosis Factor-alpha;
Vascular Endothelial Growth Factor A*
- From:The Journal of the Korean Rheumatism Association
2001;8(3):160-168
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To investigate the the effects of interleukin-17 (IL-17)on the production of vascular endothelial growth factor (VEGF)from cultured rheumatoid arthritis synoviocytes. METHODS: Fibroblast-like synovial cells(FLS)were prepared from the synovial tissues of rheumatoid arthritis patients and cultured in the presence of IL-17, IL-17 with or without transforming growth factor-beta(TGF-beta),tumor necrosis factor-alpha(TNF-alpha)and interleukin-1 beta(IL-1 beta).VEGF levels were determined in the culture supernatants by sandwitch ELISA. RESULTS: Stimulation of FLS by serial concentration of IL-17,TGF-beta,TNF-alpha,IL-1 beta increased the production of VEGF by 2.1-2.7,2.2-3.0,2.0-2.9,2.3-3.1 fold over the constitutive levels of unstimulated FLS.Stimulation of FLS by IL- 17 with TGF-beta or TNF-alpha or IL-1 beta also increased the production of VEGF accord-ing to culture periods by 1.6-1.8,1.1-1.9,1.5-1.7 fold over the levels stimulated with TGF-beta or TNF-alpha or IL-1 beta,respectively.This results indicated that IL-17 increased the effect of TGF-beta,TNF-alpha,IL-1 beta on FLS,leading synergistic enhancement of VEGF production. CONCLUSION: IL-17 may be involved in the neovascularization in rheumatoid synovitis by enhancing the production of VEGF.
