Anti-oxidation and anti-apoptosis mechanism of Xinshao formula on cerebral ischemia reperfusion injury.
10.19540/j.cnki.cjcmm.20180427.002
- Author:
Ting LIU
1
;
Fei SONG
1
;
Ding-Yan LU
1
;
Li DONG
1
;
Qiong WU
1
;
Zong-Yan LIU
1
;
Yong-Jun LI
1
;
Ai-Min WANG
2
Author Information
1. Guizhou Provincial Key Laboratory of Pharmaceutics/State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550004, China.
2. Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education), Guizhou Medical University, Guiyang 550004, China.
- Publication Type:Journal Article
- Keywords:
Xinshao formula;
apoptosis;
cerebral ischemia reperfusion injury;
reactive oxygen species
- From:
China Journal of Chinese Materia Medica
2018;43(17):3519-3524
- CountryChina
- Language:Chinese
-
Abstract:
The cerebral ischemia-reperfusion model was established to investigate the anti-oxidation and anti-apoptosis mechanism of Xinshao formula on the cerebral ischemia reperfusion injury in rats. SD rats were randomly divided into five groups: sham operation group, model group, and low, middle and high-dose Xinshao formula groups (0.31, 0.62, 1.25 g·kg⁻¹). After administration with Xinshao formula for 7 days, the rats were used to establish the cerebral ischemia-reperfusion model. The neurological behavior was evaluated. TTC staining was implemented to determine the volume of cerebral infarction. The levels of ROS, SOD, GSH-PX, NO and iNOS in serum were examined, and the mRNA and protein levels of Bcl-2, Bax and caspase 3 in hippocampal CA1 were detected by qRT-PCR, immunohistochemical assay and Western blot. It is found that Xinshao formula could significantly reduce the symptoms of nervous function and the volume of cerebral infarction in MACO rats. Compared with model group, the rats in Xinshao formula group showed increases in the activities of SOD and GSH-PX (<0.01), and decreases in the activities of iNOS and the contents of NO, ROS and MDA (<0.01). In addition, Xinshao formula could down-regulate the mRNA and protein levels of Bax and caspase 3 (<0.01), and up-regulated those of Bcl-2 (<0.01) in MACO rats. In conclusion, Xinshao formula showed aprotective effect on cerebral ischemia reperfusion injuryin rats, which may be associated with the promotion of anti-oxidation and anti-apoptosis.
