Cloning of Guamerin gene in Whitmania pigra and its spatio-temporal expression analysis after ingestion.
10.19540/j.cnki.cjcmm.20180703.003
- Author:
Man-Jun WU
1
;
Qiao-Sheng GUO
1
;
Hong-Zhuan SHI
1
;
Fei LIU
1
;
Bin WU
1
;
Guo-Wei SHI
1
;
Shi-Meng YAN
1
;
Ling GOU
1
Author Information
1. Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China.
- Publication Type:Journal Article
- Keywords:
Whitmania pigra;
human leukocyte elastase inhibitor;
ingestion;
non-bloodsucking leech;
small molecule polypeptide protein
- From:
China Journal of Chinese Materia Medica
2018;43(18):3605-3610
- CountryChina
- Language:Chinese
-
Abstract:
The present study cloned wpGuamerin gene from a non-bloodsucking leech (Whitmania pigra), and the bioinformatics analysis of the sequence was performed. Additionally, the effects of feeding duration on the expression profile of the wp Guamerin gene were explored. The results showed that its sequence consisted of 295 nucleotides encoding a peptide of 83 amino acids(Genbank: KX768545), and its relative molecular weight is 9 145.95 Da. wp Guamerin does not encode proteins with a signal peptide, belonging to the hydrophilic protein. Its secondary structure is mainly composed of α-helix, extending chain, folding and random curl. Its similarity with other blood-sucking leeches ranges from 29% to 65%. The results revealed that wpGuamerin mRNA was detected higher expression in muscle than in salivary glands of Wh. pigra, and did not expressed in ingluvies and intestine. Its expression in muscle and salivary glands showed a single peak curve after feeding and the peak was observed in the 1st and 3rd after feeding, respectively. In summary, wp Guamerin in Wh. pigra is a small molecule polypeptide protein and is different from the Guamerin in blood-sucking leeches. wpGuamerin does not express in the digestive tract of Wh. pigra, and mainly express in muscle. Feeding behavior would stimulate the expression of wpGuamerin gene in muscle and salivary glands, but not in digestive tract.
