Study on the 3,4-Dihydroxyphenylalanine Redox State Characterization Method of Mussel Adhesive Protein.

Author: Linnan KE ¹ ; Jinglong TANG ¹ ; Maoqian SONG ² ; Min GAO ² ; Yunlong LU ² ; Shuaiqi ZHAO ² ; Jingxiu BI ² ; Lizhong HE ² ; Ruihong MU ¹
Author Information

1. National Institutes for Food and Drug Control(Center for Medical Device Standardization Administration, CFDA), Beijing, 102629.
2. Jiangyin USUN Biochemical Technology Co. Ltd, Jiangyin, 214437.
Publication Type:Journal Article
Keywords: DOPA; liquid chromatography; mussel adhesive protein; redox state
From: Chinese Journal of Medical Instrumentation 2018;42(5):365-367
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the feasibility of using liquid chromatography (HPLC) to characterize the 3, 4-Dihydroxyphenylalanine (DOPA) redox state of mussel adhesive protein (MAP).
METHODSThe DOPA and protein contents of MAP were determined by HPLC, Arnow and Bradford methods respectively.
RESULTSWith extended oxidation time, the protein contents of MAP samples remained unchanged whereas the DOPA contents declined. The retention times of main peaks in HPLC for both the accelerated oxidation and retained samples shifted as the storage time extended, which could be related to the changes of sample redox state.
CONCLUSIONSThe redox state of MAP can be characterized by the change of HPLC peak retention time. HPLC can be used in the research on the MAP redox state, which is beneficial to the product development and quality control.