Impact of Mycoplasma genitalium infection on the semen quality of infertile males.

Ze-chen Yan; Xue-jun Shang; Wei Liu; Xiu-xia Wan; Chang-chun Wan; Song XU; Yong Zhong; Zhi-qiang Weng

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Impact of Mycoplasma genitalium infection on the semen quality of infertile males.

Author: Ze-Chen YAN ¹ ; Xue-Jun SHANG ² ; Wei LIU ² ; Xiu-Xia WAN ³ ; Chang-Chun WAN ⁴ ; Song XU ⁵ ; Yong ZHONG ⁶ ; Zhi-Qiang WENG ⁷
Author Information

1. Department of Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, China.
2. Department of Andrology, Nanjing School of Clinical Medicine, Southern Medical University / Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China.
3. Virtual Laboratory Section, Laboratory of Human Integrated Biological Functions, School of Basic Medicine, Qingdao University, Qingdao, Shandong 266071, China.
4. Department of Clinical Laboratory, Jinhu People's Hospital, Jinhu, Jiangsu 211600, China.
5. Department of Urology,Nanjing School of Clinical Medicine, Southern Medical University / Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China.
6. Department of Outpatients, Nanjing School of Clinical Medicine, Southern Medical University / Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China.
7. Department of Andrology, 5. Department of Urology, 6. Department of Outpatients, Nanjing School of Clinical Medicine, Southern Medical University / Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China.
Publication Type:Journal Article
Keywords: infertility; real-time fluorescence simultaneous amplification and testing; semen quality; Mycoplasma genitalium
MeSH: DNA Fragmentation; Humans; Infertility, Male; microbiology; physiopathology; Male; Male Urogenital Diseases; microbiology; Mycoplasma Infections; complications; Mycoplasma genitalium; Semen; Semen Analysis; Sperm Count; Sperm Motility; Spermatozoa; physiology
From: National Journal of Andrology 2018;24(4):317-321
CountryChina
Language:Chinese
Abstract:
ObjectiveTo explore Mycoplasma genitalium (MG) infection in the urogenital tract of infertile men and its influence on semen quality.
METHODSSemen samples were collected from 352 infertile males in the Center of Reproductive Medicine of Nanjing General Hospital from March to July 2015. MG infection was detected by real-time fluorescence simultaneous amplification and testing and semen analyses were conducted according to the WHO Laboratory Manual for the Examination and Processing of Human Semen (5th Ed) on the semen pH value, semen volume, total sperm count, sperm concentration, total sperm motility, percentages of progressively motile sperm (PMS) and immotile sperm (IMS), and sperm DNA fragmentation index (DFI). The data obtained were subjected to statistical analysis by t-test and non-parametric test (Wilcoxon test).
RESULTSMG infection was found in 3.4% (12/352) of the infertile patients. Compared with the MG-positive cases, the MG-negative ones showed a significantly higher semen volume (［2.85 ± 0.14］ vs ［3.84 ± 0.12］ ml, P = 0.008) and percentage of PMS (［15.86±1.72］ vs ［60.95 ± 5.63］ %, P = 0.032) but a lower DFI (［30.73 ±2.24］ vs ［20.71 ± 1.55］%, P = 0.014). However, no statistically significant differences were observed between the two groups in the semen pH value (7.38 ±0.02 vs 7.39 ± 0.01, P = 0.774), sperm concentration (［52.96 ± 15.78］ vs ［60.05 ± 4.29］×10⁶/ml, P = 0.683), sperm count (［154.15 ± 46.37］ vs ［221.56 ± 15.43］×106, P = 0.236), total sperm motility (［29.04 ± 3.11］ vs ［33.52 ± 1.51］ %, P = 0.626), or percentage of IMS (［23.57 ± 0.99］ vs ［62.34 ± 1.69］ %, P = 0.691).
CONCLUSIONSUrogenital MG infection is common in infertile males and potentially affects the semen quality, especially sperm vitality of the patient.