Cell suspension examination versus histopathological technique in detecting sperm in the testis tissue of non-obstructive azoospermia patients undergoing testicular sperm aspiration.

Liang Shi; Jie YU; Lai-qing Zhu; Xue Zhou; Xun Wang; Guo-hai Sun; Chun-hua Zhang

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Cell suspension examination versus histopathological technique in detecting sperm in the testis tissue of non-obstructive azoospermia patients undergoing testicular sperm aspiration.

Author: Liang SHI ¹ ; Jie YU ² ; Lai-Qing ZHU ¹ ; Xue ZHOU ¹ ; Xun WANG ¹ ; Guo-Hai SUN ¹ ; Chun-Hua ZHANG ³
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1. Department of Andrology, Drum Tower Hospital Affiliated to Nanjing University School of Medicine, Nanjing, Jiangsu 210008, China.
2. Department of Urology and Andrology, Fenjinting Hospital, Sihong, Jiangsu 223900, China.
3. Clinical Research Institute, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Publication Type:Journal Article
Keywords: cell suspension examination; histopathological technique; testicular sperm aspiration; non-obstructive azoospermia
MeSH: Azoospermia; Humans; Male; Reproductive Techniques, Assisted; Sperm Retrieval; Spermatozoa; Suspensions; Testis
From: National Journal of Andrology 2018;24(7):622-626
CountryChina
Language:Chinese
Abstract:
ObjectiveTo explore the clinical selection and application of cell suspension examination (CSE) or histopathological technique (HPT) in detecting sperm in the testis tissue obtained by testicular sperm aspiration (TESA) in patients with non-obstructive azoospermia (NOA).
METHODSTotally, 1 006 NOA patients underwent TESA and their testis tissues were subjected to CSE or HPT for sperm detection. Based on the results of CSE, the testicular tissue samples were divided into groups A (with sperm, n = 567) and B (without sperm, n = 439) and the results were compared with those of HPT.
RESULTSHPT showed 508 cases with but 59 without sperm in group A, and 403 with and 36 without sperm in group B. The consistency rate of CSE with that of HPT was 90.56% (Kappa =0.809), and CSE exhibited a significantly higher rate of sperm detection than HPT (56.36% vs 54.08%, P=0.023).
CONCLUSIONSCSE combined with HPT for detecting sperm in the testis tissue of NOA patients undergoing diagnostic TESA helps clinical diagnosis and treatment. The results of CSE have a decisive significance for assisted reproductive therapy, while those of HPT may provide some definite etiological evidence for drug therapy or surgery.