Resveratrol protects human sperm against cryopreservation-induced injury.

Shi-jia LI; Wei-dong SU; Li-jun Qiu; Xiong Wang; Juan Liu

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Resveratrol protects human sperm against cryopreservation-induced injury.

Author: Shi-Jia LI ¹ ; Wei-Dong SU ¹ ; Li-Jun QIU ¹ ; Xiong WANG ² ; Juan LIU ³
Author Information

1. Department of Clinical Laboratory, Tianjin 4th Central Hospital, Tianjin 310140, China.
2. Center of Reproductive Medicine, Yantai Yuhuangding Hospital, Yantai, Shandong 264000, China.
3. Central Laboratory, Yantai Yuhuangding Hospital, Yantai, Shandong 264000, China.
Publication Type:Journal Article
Keywords: cryopreservation; oxidative stress injury; sperm; sperm function; sperm quality; resveratrol
MeSH: Acrosome; drug effects; Animals; Antioxidants; pharmacology; Cryopreservation; methods; DNA Fragmentation; Humans; Lipid Peroxidation; Male; Malondialdehyde; Membrane Potential, Mitochondrial; Reactive Oxygen Species; analysis; Resveratrol; pharmacology; Semen Analysis; Semen Preservation; adverse effects; Sperm Motility; drug effects; Spermatozoa; drug effects; physiology
From:National Journal of Andrology 2018;24(6):499-503
CountryChina
Language:Chinese
Abstract:
ObjectiveTo investigate the effects of resveratrol in the cryopreservation medium on the quality and function of post-thaw sperm.
METHODSSemen samples were obtained from 50 normozoospermic and 50 oligoasthenozoospermic men, liquefied and then cryopreserved in the glycerol-egg yolk-citrate (GEYC) medium with or without 30 μmol/L resveratrol. Sperm motility, viability and acrosome reaction (AR) were examined before and after thawing. Sperm lipid peroxidation and the level of reactive oxygen species (ROS) were measured using commercial malondialdehyde (MDA) and the ROS assay kit. Sperm mitochondrial membrane potential (MMP) and DNA damage were determined by Rhodamine 123 staining and TUNEL.
RESULTSThe percentage of progressively motile sperm (PMS), total sperm motility, sperm viability, MMP and AR were significantly decreased (P <0.05) while the levels of sperm ROS, MDA and DNA fragmentation index (DFI) remarkably increased in both the normozoospermia and oligoasthenozoospermia groups after cryopreservation as compared with those in the fresh ejaculate (P <0.05). In comparison with the non-resveratrol control, the post-thaw sperm cryopreserved with 30 μmol/L resveratrol showed markedly higher PMS (［32.7 ± 4.8］ vs ［43.1 ± 6.3］ %, P <0.05), total motility (［44.8 ± 6.9］ vs ［56.9 ± 7.4］ %, P <0.05), viability (［52.3 ± 6.1］ vs ［67.5 ± 5.6］ %, P <0.05), MMP (［56.5 ± 7.0］ vs ［63.4 ± 7.5］ %, P <0.05) and AR (［16.6 ± 3.8］ vs ［26.3 ± 4.7］ %, P <0.05) but lower ROS, MDA and DFI (all P <0.05) in the normozoospermia group, and so did the post-thaw sperm in the oligoasthenozoospermia group, with a particularly lower DFI (［28.5 ± 4.8］ vs ［36.3 ± 5.7］%, P <0.01).
CONCLUSIONSResveratrol in the cryopreservation medium can improve the quality and function of post-thaw human sperm by reducing cryopreservation-induced sperm injury and the level of ROS.