Effects of Arsenic Trioxide on Cdc20 and Mad2 in Acute Myeloid Leukemia HL-60 Cell Line.

Shu-min Ding; Rui-rong XU; Jin-yao Kan; Yan Wang

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Effects of Arsenic Trioxide on Cdc20 and Mad2 in Acute Myeloid Leukemia HL-60 Cell Line.

Author: Shu-Min DING ¹ ; Rui-Rong XU ² ; Jin-Yao KAN ³ ; Yan WANG ⁴
Author Information

1. College of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine,Jinan 250000,Shandong Province,China.
2. Department of Hematology,The Affiliated Hospitial of Shandong University of Traditional Chinese Medicine,Jinan 250011,Shandong Province, China. E-mail: xrr18@sina.com.
3. Fourth Department of Internal Medicine,Shandong Police General Hospital, Jinan 250002, Shandong Province, China.
4. Department of Hematology,The Affiliated Hospitial of Shandong University of Traditional Chinese Medicine,Jinan 250011,Shandong Province, China.
Publication Type:Journal Article
MeSH: Antineoplastic Agents; Apoptosis; Arsenic Trioxide; Arsenicals; Cdc20 Proteins; HL-60 Cells; Humans; Leukemia, Myeloid, Acute; Oxides
From: Journal of Experimental Hematology 2018;26(3):710-715
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the effects of arsenic trioxide (AsO) on Cdc20 and Mad2 in process of AML HL-60 cell proliferation.
METHODSThe proliferation of HL-60 cells was detected by CCK-8 method at different concentrations of arsenic trioxide for 24, 48 and 72 hours. The cell morphological changes were observed by inverted microscopy. The expressions of Mad2 and Cdc20 mRNA and protein in HL-60 cells treated with AsO for 48 h were detected by real-time PCR and Western blot respectively.
RESULTSArsenic trioxide significantly inhibited the HL-60 cell proliferation and displayed a good time-dose correlation. RT-PCR and Western blot showed that the expression of Mad2 was up-regulated and the expression of Cdc20 was down-regulated in HL-60 cells treated with arsenic trioxide of different concentration (4,8,10 µmol/L).
CONCLUSIONArsenic trioxide can inhibit the human acute myeloid leukemia HL-60 cell proliferation, and its mechanism may be related with up-regulation of Mad2 expression and down-regulation of Cdc20 expression.