Effect of Blocking PYR Complex Binding to DNA Site by Peptide Nucleic Acid on γ-Globin Gene Expression.
- Author:
Si-Ying WANG
1
;
Jing LIN
2
;
Ling HUANG
1
;
Xing-Mei LIU
1
;
Yuan-Yuan HAN
1
;
Wen-Ping HAN
1
;
Bang-Quan AN
2
;
Gui-Fang LI
1
;
Sheng-Wen HUANG
3
Author Information
- Publication Type:Journal Article
- MeSH: DNA; Gene Expression; Humans; Peptide Nucleic Acids; Transfection; gamma-Globins
- From: Journal of Experimental Hematology 2018;26(3):871-874
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of blocking polypyrimidine complex binding to DNA site by using peptide nucleic acid (PNA) on γ-globin gene expression.
METHODSPYR-PNA, β-PNA and RS-PNA (random sequence-PNA) were designed and synthesized, then were transfected into K562 cells with the cationic liposome lipofectamine 2000 used as vector. The expression of γ-globin gene at both the transcriptional and translational level was detected by RT-PCR and the Western blot respectively at 24 h, 48 h and 72 h after transfection with PNAs.
RESULTSCompared with RS-PNA and control groups, the expression of γ-globin gene at mRNA and protein levels in PYR-PNA group was significantly up-regulated(P<0.05), especially at 48 h after tranfection, the levels of mRNA and protein in PYR-PNA group were increased by 2.0 and 2.5 times than those in control group, respectively.
CONCLUSIONPYR-PNA can significantly up-regulate the expression of γ-globin gene in K562 cells, this study may provide a new research idea for gene therapy of β-thalassemia.
