Effect of IKK2 Inhibitor LY2409881 on Diffuse Large B-Cell Lymphoma Cell Apoptosis and Its Mechanism.
- Author:
Pu YANG
1
;
Xiang GAO
1
;
Jun JIAO
1
;
Lin-Yan XU
1
;
Wei SANG
1
;
Xiao-Kun SUN
1
;
Kai-Lin XU
2
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Line, Tumor; Cell Proliferation; Humans; I-kappa B Kinase; Lymphoma, Large B-Cell, Diffuse; Pyrimidines; Thiophenes
- From: Journal of Experimental Hematology 2018;26(4):1086-1092
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects and its potential mechanism of IKK2 inhibitor LY2409881 on proliferation, cell cycle and apoptosis of diffuse large B-cell lymphoma (DLBCL) cell lines.
METHODSCCK8 assay was used to detect the effect of LY2409881 on proliferation of DLBCL cell lines; Flow cytometry was used to analyze cell cycle; Western blot was used to analyze apoptosis and its potential mechanism.
RESULTSLY2409881 inhibited the proliferation of multiple DLBCL cell lines obviously, and caused G cell arrest. Furtherly, LY2409881 inhibited the expression of c-FLIP, induced the activation of DR4 and caspase 8. Meanwhile, LY2409881 induced the expression of pro-apoptotic protein BAX and inhibited the expression of anti-apoptotic protein MCL-1 and BCL-2.
CONCLUSIONLY2409881 inhibits the proliferation of DLBCL cell lines, causes G cell arrest and induces apoptosis via the endogenous and exogenous apoptotic pathways.
