Effects of HDAC Inhibitor Scriptaid on IM9 Cell Line and Its Mechanism.
- Author:
Lin-Lin LIU
1
;
Xiao-Yang SUN
1
;
Yu XIE
1
;
Dan-Yang HAN
1
;
Ruo-Si YAO
1
;
Kai-Lin XU
2
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Cycle; Cell Line, Tumor; Cell Proliferation; Cyclin-Dependent Kinase Inhibitor p21; Histone Deacetylase Inhibitors; pharmacology; Humans; Hydroxylamines; pharmacology; Quinolines; pharmacology
- From: Journal of Experimental Hematology 2018;26(4):1116-1121
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of HDAC inhibitor Scriptaid on multiple myeloma IM9 cells and preliminarily clarify the mechanism of Scriptaid-induced cell apoptosis.
METHODSThe cell viability, cell cycle and cell apoptosis were measured by CCK8 assay and flow cytometry respectively, the relative target gene expression levels were detected by RT-PCR, the effect of Scriptaid on p21 promoter activity was detected by using luciferase reporter assay.
RESULTSScriptaid inhibited IM9 cell viability in a dose-dependent manner. Scriptaid induced IM9 cell cycle arrest at G/M phase in a dose-dependent manner. Scriptaid triggered IM9 cell apoptosis was obviously, the mRNA levels of apoptosis-related proteins Caspase 9, Caspase 3 and PARP1 were also activated. The apoptosis-associated factors BAD, PTEN and p21 increased following treatment with different dose of Scriptaid, meanwhile, p21 promoter activity was also activated significantly.
CONCLUSIONHDAC inhibitor Scriptaid can promote IM9 cell apoptosis by transcriptional activation of p21 promoter in concentration-dependent manner.
