Detection of HHV-8 Virus in 450 Free Blood Donons in Wuhan and Its Genotyping Based on ORFK1 Gene.
- Author:
Rong FU
1
;
Jin YU
2
;
Ming-Zhen JING
2
;
Xu-Ying WANG
2
Author Information
- Publication Type:Journal Article
- MeSH: Blood Donors; DNA, Viral; Genotype; Herpesvirus 8, Human; Phylogeny; Polymerase Chain Reaction
- From: Journal of Experimental Hematology 2018;26(4):1198-1203
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the infection of HHV-8 virus in free blood donors in Wuhan.
METHODSWhole blood samples were collected from 450 free blood donors in Wuhan, and the genomic DNA extraction and serum collection were performed respectively. And then, the positive rate of HHV-8 was detected by PCR and ELISA, the positive detection rates were compared between them. Finally, HHV-8 ORFK1 gene was cloned by PCR in the positive samples, and the HHV-8 ORFK1 gene th was genetyped through sequencing analysis, homology comparison and phylogenetic tree construction.
RESULTS25 and 23 cases of positive samples were detected by PCR and ELISA, their positive rate were 5.56% and 5.11% respectively, and without statistically significant difference using χ test analysis (P > 0.05). Based on the results of ORFK1 gene cloning and sequence analysis in 23 positive samples, 15 samples C subtype of had HHV-8 ORFK1 gene, and 8 cases had A subtype had HHV-8 ORFK1 gene.
CONCLUSIONThere is a certain percentage of HHV-8 infection in the free blood donors in Wuhan. It is suggested to increase the HHV-8 virus screening for free blood donors, so as to ensure the quality of blood.
