Advances of studies on Purification and Tracking of Hematopoietic Stem Cells Using Their Specific Gene Expression--Review.
10.7534/j.issn.1009-2137.2018.04.045
- Author:
Min-Er XIE
1
;
Fang DONG
1
;
Tao CHENG
2
,
3
,
4
;
Ema HIDEO
2
,
3
,
5
Author Information
1. State Key Laboratory of Experimental Hematology, Institute of Hematology& Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020,China.
2. State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020
3. Center for Stem Cell Medicine, Chinese Academy of Medical Sciences, Tianjin 300020
4. Department of Stem Cell and Regenerative Medicine, Peking Union Medical College, Tianjin 300020, China.
5. Department of Stem Cell and Regenerative Medicine, Peking Union Medical College, Tianjin 300020, China .E-mail: hema@ihcams.ac.cn.
- Publication Type:Journal Article
- MeSH:
Cell Differentiation;
Flow Cytometry;
Gene Expression;
Hematopoiesis;
Hematopoietic Stem Cells
- From:
Journal of Experimental Hematology
2018;26(4):1215-1219
- CountryChina
- Language:Chinese
-
Abstract:
Hematopoietic stem cells (HSC) maintain homeostatic hematopoiesis via their multi-lineage differentiation and self-renewal potentials. HSC can be enriched and purified by flow cytometry relying on their cell surface markers and functional characteristics, however, these methods can not meet the need for deep analysis of HSC biological property and function because of the poor purity. Recent studies have successfully purified and tracked HSC using specifically expressed genes, which can enhance the purification efficiency of HSC, thus provide a better tool for the in-vivo study of HSC. This review summarizes the new techniques and discusses their advantages and disadvantages.
