Apoptosis-related mRNA expression profiles of ovarian cancer cell lines following cisplatin treatment.
10.3802/jgo.2010.21.4.255
- Author:
JooHee YOON
1
;
Eung Sam KIM
;
Sung Jong LEE
;
Chang Wook PARK
;
Hyung Jin CHA
;
Bee Hak HONG
;
Kwan Yong CHOI
Author Information
1. Department of Obstetrics and Gynecology, St. Vincent's Hospital, The Catholic University of Korea, Suwon, Korea.
- Publication Type:Original Article
- Keywords:
Ovarian cancer cell lines;
Cisplatin;
Apoptosis;
Dendron-modified DNA microarray
- MeSH:
Apoptosis;
Cell Death;
Cell Line;
Cisplatin;
DNA;
Flow Cytometry;
Inhibitory Concentration 50;
Ovarian Neoplasms;
RNA, Messenger;
Up-Regulation
- From:Journal of Gynecologic Oncology
2010;21(4):255-261
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: The aim of this study was to identify apoptosis-related genes of ovarian cancer cell lines following cisplatin treatment. METHODS: We used IC50 values and fluorescence-activated cell sorting analysis to compare cell death in 2 ovarian cancer cell lines, namely, SKOV-3 and OVCAR-3, upon treatment with cisplatin. Moreover, the change in transcriptional levels of apoptosis-associated genes was measured with a dendron-modified DNA microarray. RESULTS: The protein levels for the up-regulated genes in each cell line were validated to identify the molecules that may determine the cellular behavior of cisplatin resistance. Eight genes were over-expressed in the 2 cell lines. The cisplatin-induced up-regulation of DAD1 in transcriptional and protein levels contributed to the cisplatin resistance of OVCAR-3, and the up-regulation of FASTK and TNFRSF11A in SKOV-3 resulted in its higher sensitivity to cisplatin than that of OVCAR-3. CONCLUSION: In the present study, we have identified a set of genes responsible for apoptosis following cisplatin treatment in ovarian cancer cell lines. These genes may give information about the understanding of cisplatin-induced apoptosis in ovarian cancer.
