Hepatopulmonary Syndrome Induced by Common Bile Duct Ligation in a Rabbit Model: Correlation between Pulmonary Vascular Dilatation on Thin-Section CT and Angiography and Serum Nitrite Concentration or Endothelial Nitric Oxide Synthase (eNOS) 1 Expression.
- Author:
Ki Nam LEE
1
;
Seong Kuk YOON
;
Jin Wha LEE
;
Ki Nam KIM
;
Byung Ho PARK
;
Jong Young KWAK
;
Jin Sook JEONG
;
Young Hoon KIM
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: Liver, injuries; Lung, angiography; Lung, CT; Lung, injuries
- MeSH: Angiography; Animals; Common Bile Duct/injuries; Dilatation, Pathologic/radiography; Disease Models, Animal; Endothelium, Vascular/metabolism; Hepatopulmonary Syndrome/etiology/*metabolism; Ligation; Lung/*blood supply/metabolism/*radiography; Nitric-Oxide Synthase/*metabolism; Nitrites/*blood; Pulmonary Artery/radiography; Rabbits; Research Support, Non-U.S. Gov't; Tomography, X-Ray Computed/methods
- From:Korean Journal of Radiology 2004;5(3):149-156
- CountryRepublic of Korea
- Language:English
- Abstract: OBJECTIVE: To investigate the correlation between radiologic vascular dilatation and serum nitrite concentration and eNOS expression in the endothelial cell and pneumocyte in a rabbit model of hepatopulmonary syndrome induced by common bile duct ligation (CBDL). MATERIALS AND METHODS: Thin-section CT scans of the lung and pulmonary angiography were obtained 3 weeks after CBDL (n=6), or a sham operation (n=4), and intrapulmonary vasodilatation was assessed. The diameter and tortuosity of peripheral vessels in the right lower lobe by thin-section CT and angiography at the same level of the right lower lobe in all subjects were correlated to serum nitrite concentration and eNOS (endothelial nitric oxide synthase) expression as determined by immunostaining. RESULTS: The diameters of pulmonary vessels on thin-section CT were well correlated with nitrite concentrations in serum (r = 0.92, p < 0.001). Dilated pulmonary vessels were significantly correlated with an increased eNOS expression (r = 0.94, p < 0.0001), and the severity of pulmonary vessel tortuosity was found to be well correlated with serum nitrite concentration (r = 0.90, p < 0.001). CONCLUSION: The peripheral pulmonary vasculature in hepatopulmonary syndrome induced by CBLD was dilated on thin-section CT and on angiographs. Our findings suggest that peripheral pulmonary vascular dilatations are correlated with serum nitrite concentrations and pulmonary eNOS expression.
