Reduced Number of Endothelial Progenitor Colony-Forming Units in Patients with Preeclampsia.
- Author:
Shin Young KIM
1
;
So Yeon PARK
;
Jin Woo KIM
;
Mi Bum LEE
;
You Jung HAN
;
Hyun Kyong AHN
;
Jun Seek CHOI
;
Jung Yeol HAN
;
Moon Young KIM
;
Kyu Hong CHOI
;
Hyun Mee RYU
Author Information
1. Laboratory of Medical Genetics, Cheil General Hospital and Women's Healthcare Center, Kwandong University College of Medicine, Seoul, Korea. hmryu@yahoo.com
- Publication Type:Original Article
- Keywords:
Preeclampsia;
Endothelial progenitor cells;
Colony-forming unit;
VEGF;
sFlt-1;
PlGF
- MeSH:
Angiogenesis Inducing Agents;
Endometrium;
Female;
Humans;
Lipoproteins;
Plasma;
Pre-Eclampsia;
Pregnancy;
Pregnancy Trimester, Third;
Stem Cells;
Tyrosine;
Vascular Diseases;
Vascular Endothelial Growth Factor A
- From:Journal of Genetic Medicine
2010;7(2):138-144
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Endothelial progenitor cells (EPCs), which mediates neovascularization of uterine endometrium may be involved in the neovascularization in the utero-placental circulation. Low numbers of endothelial progenitor colony-forming unit (CFU) in culture are predictive biomarker of vascular disease. The aim of the present study was to evaluate whether the number of CFU in preeclampsia differed from that in normal pregnancy. MATERIALS AND METHODS: Women with singleton normal (n=26) or preeclamptic (n=20) pregnancies were studied during the third trimester. The number of EPCs was quantified by CFU methodology. Plasma levels of angiogenic factors, vascular endothelial growth factor (VEGF), soluble fms-like tyrosine kinase-1 (sFlt-1), and placental growth factor (PlGF) were determined by enzyme-linked immunoassay. RESULTS: CFU numbers were significantly decreased in the preeclamptic patients compared with the controls (median, 3; range 1-12 vs. 31; 3-81 CFU/well, P<0.001). A majority of the cells comprising individual colonies were positive for endothelial characteristics (Ulex europaeus lectin staining and acetylated low-density lipoprotein uptake). Plasma levels of the sFlt-1 were highly elevated (P<0.001) in patient with preeclampsia compared to controls, whereas PlGF were highly reduced (P=0.004), but these factors did not associate with CFU numbers. CONCLUSION: Our results suggest that reduced numbers of CFU obtained from maternal peripheral blood may contribute to the development of preeclampsia.
