Inhibition of the Hedgehog Signaling Pathway Depresses the Cigarette Smoke-Induced Malignant Transformation of 16HBE Cells on a Microfluidic Chip.

Yong-xin Qin; Zhi-hui Yang; Xiao-hui DU; Hui Zhao; Yuan-bin Liu; Zhe Guo; Qi Wang

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Inhibition of the Hedgehog Signaling Pathway Depresses the Cigarette Smoke-Induced Malignant Transformation of 16HBE Cells on a Microfluidic Chip.

Author: Yong-Xin QIN ^{1
,

2} ; Zhi-Hui YANG ³ ; Xiao-Hui DU ³ ; Hui ZHAO ⁴ ; Yuan-Bin LIU ⁴ ; Zhe GUO ⁴ ; Qi WANG ⁴
Author Information

1. Department of Respiratory Medicine, The Second Affiliated Hospital, Dalian Medical University
2. Department of Critical Care Medicine, The First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning 116021, China.
3. Department of Scientific Research Center, The Second Affiliated Hospital, Dalian Medical University, Dalian, Liaoning 116027, China.
4. Department of Respiratory Medicine, The Second Affiliated Hospital, Dalian Medical University, Dalian, Liaoning 116027, China.
Publication Type:Journal Article
Keywords: Hedgehog Signaling System; Lung Cancer; Malignant Transformation; Microfluidic Chip
MeSH: Animals; Cell Transformation, Neoplastic; genetics; metabolism; Gene Expression Regulation, Neoplastic; genetics; physiology; Hedgehog Proteins; genetics; metabolism; Lab-On-A-Chip Devices; Mice; Mice, Inbred BALB C; Mice, Nude; Microfluidics; Signal Transduction; genetics; physiology; Smoke; Smoking; adverse effects
From: Chinese Medical Journal 2018;131(10):1191-1198
CountryChina
Language:English
Abstract:
BackgroundThe hedgehog signaling system (HHS) plays an important role in the regulation of cell proliferation and differentiation during the embryonic phases. However, little is known about the involvement of HHS in the malignant transformation of cells. This study aimed to detect the role of HHS in the malignant transformation of human bronchial epithelial (16HBE) cells.
MethodsIn this study, two microfluidic chips were designed to investigate cigarette smoke extract (CSE)-induced malignant transformation of cells. Chip A contained a concentration gradient generator, while chip B had four cell chambers with a central channel. The 16HBE cells cultured in chip A were used to determine the optimal concentration of CSE for inducing malignant transformation. The 16HBE cells in chip B were cultured with 12.25% CSE (Group A), 12.25% CSE + 5 μmol/L cyclopamine (Group B), or normal complete medium as control for 8 months (Group C), to establish the in vitro lung inflammatory-cancer transformation model. The transformed cells were inoculated into 20 nude mice as cells alone (Group 1) or cells with cyclopamine (Group 2) for tumorigenesis testing. Expression of HHS proteins was detected by Western blot. Data were expressed as mean ± standard deviation. The t-test was used for paired samples, and the difference among groups was analyzed using a one-way analysis of variance.
ResultsThe optimal concentration of CSE was 12.25%. Expression of HHS proteins increased during the process of malignant transformation (Group B vs. Group A, F = 7.65, P < 0.05). After CSE exposure for 8 months, there were significant changes in cellular morphology, which allowed the transformed cells to grow into tumors in 40 days after being inoculated into nude mice. Cyclopamine could effectively depress the expression of HHS proteins (Group C vs. Group B, F = 6.47, P < 0.05) and prevent tumor growth in nude mice (Group 2 vs. Group 1, t = 31.59, P < 0.01).
ConclusionsThe activity of HHS is upregulated during the CSE-induced malignant transformation of 16HBE cells. Cyclopamine can effectively depress expression of HHS proteins in vitro and prevent tumor growth of the transformed cells in vivo.