Human Leukocyte Antigen-A Allele Distribution in Nasopharyngeal Carcinoma Patients Showing Anti-Melanoma-Associated Antigen A or Synovial Sarcoma X-2 T Cell Response in Blood.

Pei-wen Fan; Li Huang; Xue-mei Chang; Ya-ning Feng; Xuan Yao; Yan-chun Peng; Tao Dong; Ruo-zheng Wang

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Human Leukocyte Antigen-A Allele Distribution in Nasopharyngeal Carcinoma Patients Showing Anti-Melanoma-Associated Antigen A or Synovial Sarcoma X-2 T Cell Response in Blood.

Author: Pei-Wen FAN ¹ ; Li HUANG ² ; Xue-Mei CHANG ¹ ; Ya-Ning FENG ¹ ; Xuan YAO ^{3
,

4} ; Yan-Chun PENG ^{3
,

4} ; Tao DONG ^{3
,

4} ; Ruo-Zheng WANG ^{5
,

6}
Author Information

1. Xinjiang Key Laboratory of Oncology, The Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830000, China.
2. Department of Radiation Oncology, The Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830000, China.
3. CAMS Oxford Center for Translation Immunology, Chinese Academy of Medical Science Oxford Institute, Nuffield Department of Medicine
4. MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, Oxford University, Oxford OX3 9DS, UK.
5. Xinjiang Key Laboratory of Oncology, The Affiliated Tumor Hospital of Xinjiang Medical University
6. Department of Radiation Oncology, The Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830000, China.
Publication Type:Journal Article
Keywords: Human Leukocyte Antigen-A; Melanoma-Associated Antigen-A; Nasopharyngeal Carcinoma; Synovial Sarcoma X-2; T Cell Response
MeSH: Adolescent; Adult; Aged; Aged, 80 and over; Alleles; Antigens, Neoplasm; immunology; metabolism; Carcinoma; immunology; metabolism; Female; HLA-A Antigens; metabolism; Humans; Leukocytes, Mononuclear; metabolism; Male; Middle Aged; Nasopharyngeal Carcinoma; Nasopharyngeal Neoplasms; immunology; metabolism; Neoplasm Proteins; metabolism; Sarcoma, Synovial; immunology; metabolism; Young Adult
From: Chinese Medical Journal 2018;131(11):1289-1295
CountryChina
Language:English
Abstract:
BackgroundDevelopment of innovative immunotherapy is imperative to improve the poor survival of the nasopharyngeal carcinoma (NPC) patients. In this study, we evaluated the T cell response to melanoma-associated antigen (MAGE)-A1, MAGE-A3, or synovial sarcoma X-2 (SSX-2) in the peripheral blood of treatment-naive NPC patients. The relationship of responses among the three proteins and the human leukocyte antigen (HLA)-A types were analyzed to provide evidence of designing novel therapy.
MethodsSixty-one NPC patients admitted into the Tumor Hospital affiliated to the Xinjiang Medical University between March 2015 and July 2016 were enrolled. Mononuclear cells were isolated from the peripheral blood before any treatment. HLA-A alleles were typed with Sanger sequence-based typing technique. The T cell response to the MAGE-A1, MAGE-A3, or SSX-2 was evaluated with the Enzyme-Linked ImmunoSpot assay. Mann-Whitney U-test was used to compare the T cell responses from different groups. Spearman's rank correlation was used to analyze the relationship of T cell responses.
ResultsHLA-A*02:01, A*02:07, and A*24:02 were the three most frequent alleles (18.9%, 12.3%, and 11.5%, respectively) among the 22 detected alleles. 31.1%, 19.7%, and 16.4% of the patients displayed MAGE-A1, MAGE-A3, or SSX-2-specific T cell response, respectively. The magnitudes of response to the three proteins were 32.5, 38.0, and 28.7 SFC/10 peripheral blood mononuclear cells, respectively. The T cell response against the three proteins correlated with each other to different extent. The percentage of A*02:01 and A*24:02 carriers were significantly higher in patients responding to any of the three proteins compared to the nonresponders.
ConclusionMAGE-A1, MAGE-A3, or SSX-2-specific T cell responses were detectable in a subgroup of NPC patients, the frequency and magnitude of which were correlated.