Preparation of multifunctional nanoparticles targeting tongue cancer and in vitro study.

Wei REN; Li-Hua QIU; Zhi GAO; Pan LI; Xin ZHAO; Cheng-Chen HU

Return

Preparation of multifunctional nanoparticles targeting tongue cancer and in vitro study.

VernacularTitle:靶向舌癌多功能纳米粒的制备和体外实验
Author: Wei REN ¹ ; Li-Hua QIU ^{2
,

3} ; Zhi GAO ⁴ ; Pan LI ⁵ ; Xin ZHAO ⁶ ; Cheng-Chen HU ¹
Author Information

1. Dep. of Oral and Maxillofacial Surgery, Stomatological Hospital of Chongqing Medical University, Chongqing Key Laboratory of Oral Diseases and Biomedical Science, Chongqing 401147, China.
2. Dep. of Oral and Maxillofacial Surgery, Stomatological Hospital of Chongqing Medical University, Chongqing Key Laboratory of Oral Diseases and Biomedical Science, Chongqing 401147, China
3. Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China.
4. Dept. of Stomatology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
5. Institute of Ultrasound Imaging, Chongqing Medical University, Chongqing 400010, China.
6. Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China.
Publication Type:Journal Article
Keywords: indocyanine green; molecular imaging; poly (lactic-co-glycolic acid); targeted therapy; tongue carcinoma
From: West China Journal of Stomatology 2018;36(3):240-246
CountryChina
Language:Chinese
Abstract:
OBJECTIVEThis study aims to prepare docetaxel (DOC)-loaded multifunctional nanoparticles containing indocyanine green (ICG) and perfluorohexane (PFH) as targeted drug delivery system, which is supplemented with stromal cellderived factor-1 (SDF-1), and characterize their properties.
METHODSMultifunctional nanoparticles were prepared by using the double emulsion method. SDF-1 was covalently conjugated to the surface of the nanoparticles through thioether bonding. Their particle size, distribution, and surface potential were determined with the Malvern measuring instrument. The conjugation of SDF-1 was evaluated by confocal laser scanning microscope. Encapsulation efficiency (ELC), drug loading capacity (DLC), and release regularity of the nanoparticles were determined by high-performance liquid chromatography (HPLC). In vitro photothermal property was recorded by a thermal imager. The in vitro imaging capacity was observed by a photoacoustic instrument and an ultrasonic diagnostic apparatus. Targeting capability was assessed by flow cytometry. The cell activity on SCC-15 cells was checked by CCK-8 method.
RESULTSThe targeted multifunctional nanoparticles showed regularly sphericity. The diameter was (502.88±17.92) nm. The zeta potential was (-11.5±3.15) mV. ELC was 54.12%±1.74%. DLC was 1.08 mg·mL-1. In vitro drug release was initially fast and subsequently slow. The photothermal characteristics were related to the concentration; the higher the concentration, the higher the temperature. Nanoparticles could detect significant photoacoustic and ultrasound signals. The in vitro targeting rate was 89.99%. No significant differences of cell viability in the SINPs groups were observed at each concentration (P>0.05). The inhibition effect of DOC-SINPs was stronger than that of SINPs whether or not in the presence of laser irradiation among the groups of 150 and 200 μg·mL-1 (P< 0.05).
CONCLUSIONSMultifunctional nanoparticles for diagnosis and treatment were successfully prepared and displayed dualmode ultrasound/photoacoustic imaging and antitumor effects of chemotherapy and photothermal therapy.