An in vitro study of the angiogenic effects of concentrate growth factor on human umbilical vein endothelial cells.

Jun HUAN; Lei DOU; Qi-Fang YAN; De-Qin YANG

Return

An in vitro study of the angiogenic effects of concentrate growth factor on human umbilical vein endothelial cells.

VernacularTitle:浓缩生长因子促人脐静脉血管内皮细胞成血管化作用研究
Author: Jun HUAN ^{1
,

2
,

3} ; Lei DOU ^{1
,

2
,

3} ; Qi-Fang YAN ^{1
,

2
,

3} ; De-Qin YANG ^{1
,

2
,

3}
Author Information

1. Dept. of Conservative Dentistry and Endodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China
2. Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, China
3. Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China.
Publication Type:Journal Article
Keywords: concentrate growth factor; differentiation; human umbilical vein endothelial cells; proliferation
From: West China Journal of Stomatology 2018;36(3):247-251
CountryChina
Language:Chinese
Abstract:
OBJECTIVEThis study aimed to explore the effects of concentrate growth factor extracts (CGFe) on human umbilical vein endothelial cells (HUVECs) in vitro.
METHODSConcentrate growth factor (CGF) were prepared from the peripheral blood of healthy donors, followed by CGFe. Four groups were designed based on cell culture medium, as follows: 2%CGFe, 5%CGFe, 10%CGFe, and control. The proliferation activity of HUVECs was detected by cell cycle and CCK-8 assays. The migration of HUVECs was detected by scratch assay. The mRNA expression levels of vascular endothelial growth factor (VEGF), chemokine receptor 4 (CXCR4), and platelet derived growth factor (PDGF) were examined by quantitative real time polymerase chain reaction (qRT-PCR).
RESULTSResults of CCK-8 and cell cycle assays showed that CGFe promoted the proliferation capability of HUVECs in a dose-dependent manner, and the data had statistical significance among four groups (P< 0.05). The cell migration assay indicated that CGF accelerated wound closure in a dose-dependent manner after 12 h of culture (P<0.05). The results of qRT-PCR showed that CGF upregulated the expression levels of VEGF, CXCR4, and PDGF in HUVECs.
CONCLUSIONSCGFe can promote the proliferation, migration, and angiogenic differentiation of HUVECs. Thus, CGF might be an appropriate cure for dental pulp revascularization.