Link between sortase A function and cariogenicity of Streptococcus mutans: a preliminary metabolomics analysis.

Ying SONG; Jing-Lin ZHOU; Yuan-Li HE; Wei LI; Ling ZOU

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Link between sortase A function and cariogenicity of Streptococcus mutans: a preliminary metabolomics analysis.

Author: Ying SONG ^{1
,

2} ; Jing-Lin ZHOU ³ ; Yuan-Li HE ⁴ ; Wei LI ⁴ ; Ling ZOU ⁵
Author Information

1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
2. Dept. of Conservative Dentistry and Endodontics, Stomatological Hospital of Chongqing Medical University, Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences,Chongqing 401147, China.
3. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
4. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
5. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Conservative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Publication Type:Journal Article
Keywords: Streptococcus mutans; metabonomics; orthogonality partial least squares discrimination analysis; principal component analysis; sortase A; ¹H nuclear magnetic resonance spectroscopy
From: West China Journal of Stomatology 2018;36(4):360-366
CountryChina
Language:Chinese
Abstract:
OBJECTIVEThis study intends to explore the mechanism underlying the support of sortase A (SrtA) of the cariogenicity of Streptococcus mutans (S. mutans).
METHODSWe performed a metabonomics study based on ¹H nuclear magnetic resonance spectroscopy (NMR), in which we compared the extracellular metabolites of wild-type S. mutans UA159 with those of its SrtA-deficient strain. Metabolite differences among strains were identified using a combination of principal component analysis and orthogonality partial least square discriminant analysis.
RESULTSSeveral differences corresponding mostly to unknown metabolites were identified. Some amino acids such as leucine and valine (δ 0.92×10⁻⁶-1.20×10⁻⁶), lactic acid ( δ1.28×10⁻⁶), oxoglutaric acid (δ 3.00×10⁻⁶), and glycine (δ 3.60×10⁻⁶) differed among strains.
CONCLUSIONSThis work establishes the feasibility of using ¹H NMR-based metabonomics to provide leads for research into molecular factors that promote caries. The database of microbial metabolites should be also improved in further studies.